DDX1 Antibody (3E5B2) - BSA Free

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Western Blot: DDX1 Antibody (3E5B2) [NBP2-61744] - Analysis using DDX1 mAb against HEK293 (1) and DDX1 (AA: 642-740)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: DDX1 Antibody (3E5B2) [NBP2-61744] - Analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry: DDX1 Antibody (3E5B2) [NBP2-61744] - Analysis of paraffin-embedded esophageal cancer tissues using DDX1 mouse mAb with DAB staining.
Flow Cytometry: DDX1 Antibody (3E5B2) [NBP2-61744] - Analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).
Western Blot: DDX1 Antibody (3E5B2) [NBP2-61744] - Analysis using DDX1 mAb against human DDX1 (AA: 642-740) recombinant protein. (Expected MW is 37 kDa)
Western Blot: DDX1 Antibody (3E5B2) [NBP2-61744] - Analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), and Jurkat (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: DDX1 Antibody (3E5B2) [NBP2-61744] - Analysis of Hela cells using DDX1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 ...read more
Immunohistochemistry: DDX1 Antibody (3E5B2) [NBP2-61744] - Analysis of paraffin-embedded cervical cancer tissues using DDX1 mouse mAb with DAB staining.
ELISA: DDX1 Antibody (3E5B2) [NBP2-61744] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
3E5B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

DDX1 Antibody (3E5B2) - BSA Free Summary

Immunogen
Purified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DDX1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Theoretical MW
82.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for DDX1 Antibody (3E5B2) - BSA Free

  • ATP-dependent RNA helicase DDX1
  • DBP-RB
  • DBP-RBDEAD box polypeptide 1
  • DDX1
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
  • DEAD box protein 1
  • DEAD box protein retinoblastoma
  • DEAD Box-1
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
  • DEAD/H-Box Helicase 1
  • EC 3.6.1
  • EC 3.6.1.10
  • EC 3.6.4.13
  • UKVH5d

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DDX1