DDR1 Antibody - BSA Free

Western Blot: DDR1 Antibody [NBP1-33134] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:5000.

Immunohistochemistry-Paraffin: DDR1 Antibody [NBP1-33134] - Human ovarian cancer, using DDR1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: DDR1 Antibody [NBP1-33134] - Mouse colon.DDR1 stained by DDR1 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: DDR1 Antibody [NBP1-33134] -Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR1 antibody [N1N3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR1 antibody [N1N3] (NBP1-33134) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DDR1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DDR1 Antibody - BSA Free (NBP1-33134) is a polyclonal antibody validated for use in IHC and WB. Anti-DDR1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DDR1. The exact sequence is proprietary.
• Localization: Isoform 1: Membrane, Isoform 2: Membrane, Isoform 3: Secreted, Isoform 4: Secreted
• Predicted Species: Rat (91%), Porcine (95%), Chimpanzee (100%), Bovine (92%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDR1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 101 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for DDR1 Antibody - BSA Free

• CAK
• CD167 antigen-like family member A
• CD167a antigen
• CD167a
• DDR
• DDR1
• discoidin domain receptor family, member 1
• discoidin domain receptor tyrosine kinase 1
• Discoidin receptor tyrosine kinase
• EC 2.7.10
• EC 2.7.10.1
• EDDR1
• ENTRK4
• Epithelial discoidin domain receptor 1
• HGK2
• MCK10
• MCK-10
• NTRK4
• Protein-tyrosine kinase 3A
• PTK3A protein tyrosine kinase 3A
• PTK3A
• receptor, type 4
• RTK6
• TRK E
• TrkE

Background

discoidin domain receptor tyrosine kinase 1. Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DDR1 Antibody (NBP1-33134)(2)

Publications using NBP1-33134

• Torun YM, Delen E, Doganlar O et al. Effects of Expression of Matrix Metalloproteinases and Discoidin Domain Receptors in Ligamentum Flavum Fibrosis in Patients with Degenerative Lumbar Canal Stenosis Asian spine journal 2022-09-27 [PMID: 36163678] (WB, Human)
• Tao Y, Wang R, Lai Q Targeting of DDR1 with antibody-drug conjugates has antitumor effects in a mouse model of colon carcinoma Mol Oncol 2019-05-22 [PMID: 31116512] (IHC-P, Human, Mouse)

FAQs for DDR1 Antibody (NBP1-33134). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you offer, or are planning on offering, any antibodies targeted to specific phosphorylation sites on the receptor tyrosine kinase DDR1.
   • At this time we do not offer and DDR1 antibodies specific for certain phosphorylation states.

Bioinformatics

• Gene Symbol: DDR1