Human DC-SIGN/CD209 ELISA Kit (Colorimetric)

ELISA: Human DC-SIGN/CD209 ELISA Kit (Colorimetric) [NBP2-80334] - Example standard curve for Human DC-SIGN/CD209 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Discontinued

Human DC-SIGN/CD209 ELISA Kit (Colorimetric) Summary

• Specificity: Recognizes both recombinant and natural Human DC-SIGN/CD209.
• Standard Curve Range: 31.25-2000 pg/ml (example only; lot dependent)
• Sensitivity: 11.8 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 8 %
• Intra-Assay: CV% < 4.1
• Spike Recovery: 109%-114%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CD209

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Alternate Names for Human DC-SIGN/CD209 ELISA Kit (Colorimetric)

• CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
• CD209 molecule
• CD209
• CDSIGNHIV gpl20-binding protein
• CLEC4L
• CLEC4LC-type lectin domain family 4 member L
• DCSIGN
• DC-SIGN
• DC-SIGN1
• DC-SIGN1C-type lectin domain family 4, member L
• DC-SIGNMGC129965
• Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Background

DC-SIGN encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for DC-SIGN/CD209 ELISA Kit (NBP2-80334). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CD209