DC-SIGN/CD209 Antibody (902404) [Phycoerythrin]

• Applications: Flow
• Clone: 902404
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

DC-SIGN/CD209 Antibody (902404) [Phycoerythrin] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: NS0 mouse myeloma cell line transfected with mouse DC‑SIGN/CD209
  Met1-Lys238
  Accession # Q91ZX1
• Marker: Dendritic Cell Marker
• Specificity: Detects mouse DC‑SIGN/CD209 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CD209
• Purity Statement: Protein A or G purified from cell culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Mouse Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209, also known as CD209 Antigen-like Protein A, is a member of the C-type lectin family (1). Mouse DC-SIGN/CD209 is a 33 kDa, 238 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca²⁺-dependent carbohydrate-binding lectin domain (2). In addition to the full-length mouse DC-SIGN/CD209, a second, truncated splice variant has been reported. DC-SIGN/CD209 is not well conserved between mouse and human with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2-4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (2, 5, 6).

1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
2. Caminschi, I. et al. (2001) Mol. Immunol. 38:365.
3. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD209