DC-SIGN/CD209 Antibody (5C2A6) - BSA Free

Western Blot: DC-SIGN/CD209 Antibody (5C2A6) [NBP2-61741] - Analysis using CD209 mAb against HEK293 (1) and CD209 (AA: extra 270-404)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: DC-SIGN/CD209 Antibody (5C2A6) [NBP2-61741] - Analysis of Ramos cells using CD209 mouse mAb (green) and negative control (red).

Western Blot: DC-SIGN/CD209 Antibody (5C2A6) [NBP2-61741] - Analysis using CD209 mAb against human CD209 (AA: extra 270-404) recombinant protein. (Expected MW is 41.1 kDa)

Western Blot: DC-SIGN/CD209 Antibody (5C2A6) [NBP2-61741] - Analysis using CD209 mouse mAb against Hela (1), U937 (2), THP-1 (3), HL-60 (4), and A431 (5) cell lysate.

ELISA: DC-SIGN/CD209 Antibody (5C2A6) [NBP2-61741] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Discontinued

DC-SIGN/CD209 Antibody (5C2A6) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human CD209 (AA: extra 270-404) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD209
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:100-1:2000
• Theoretical MW: 45.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for DC-SIGN/CD209 Antibody (5C2A6) - BSA Free

• CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
• CD209 molecule
• CD209
• CDSIGNHIV gpl20-binding protein
• CLEC4L
• CLEC4LC-type lectin domain family 4 member L
• DCSIGN
• DC-SIGN
• DC-SIGN1
• DC-SIGN1C-type lectin domain family 4, member L
• DC-SIGNMGC129965
• Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Background

This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD209