DC-LAMP Antibodies

204-IL) and 50 ng/mL Recombinant Human GM-CSF (215-GM) for 7 days and 1 µg/mL LPS, 20 ng/mL Recombinant Human TNF-a (210-TA), and 20 ng/mL Recombinant Human IL-1 beta /IL-1F2 (201-LB) for last 24 hours were stained with Goat Anti-Human DC-LAMP Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4087, filled histogram) or isotype control (AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Allophycocyanin (F0108)." class="big_lightbox"> 204-IL) and 50 ng/mL Recombinant Human GM-CSF (215-GM) for 7 days and 1 µg/mL LPS, 20 ng/mL Recombinant Human TNF-a (210-TA), and 20 ng/mL Recombinant Human IL-1 beta /IL-1F2 (201-LB) for last 24 hours were stained with Goat Anti-Human DC-LAMP Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4087, filled histogram) or isotype control (AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Allophycocyanin (F0108)." alt="Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14⁺cells treated with 20 ng/mL Recombinant Human IL-4 (204-IL) and 50 ng/mL Recombinant Human GM-CSF (215-GM) for 7 days and 1 µg/mL LPS, 20 ng/mL Recombinant Human TNF-a (210-TA), and 20 ng/mL Recombinant Human IL-1 beta /IL-1F2 (201-LB) for last 24 hours were stained with Goat Anti-Human DC-LAMP Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4087, filled histogram) or isotype control (AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Allophycocyanin (F0108)." class="big_thumb" />

HAF007). A specific band was detected for DC-LAMP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for DC-LAMP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates ofin vitrodifferentiated human myeloid mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DC-LAMP Monoclonal Antibody (Catalog # MAB4087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DC-LAMP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />