OBCAM/OPCML Antibody [Unconjugated]

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OBCAM/OPCML was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human OBCAM/OPCML Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2777) at 10 µg/mL for 3 hours at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

OBCAM/OPCML Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human OBCAM/OPCML
Gly28-Asn322
Accession # Q14982
Specificity
Detects human OBCAM/OPCML in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) KILON is observed and less than 1% cross-reactivity with rhLAMP is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OPCML
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publication using
AF2777 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OBCAM/OPCML Antibody [Unconjugated]

  • IgLON family member 1
  • IGLON1opioid-binding protein/cell adhesion molecule-like
  • OBCAM
  • OBCAMopiate binding-cell adhesion molecule
  • OPCM
  • OPCML
  • opioid binding protein/cell adhesion molecule-like preprotein
  • opioid binding protein/cell adhesion molecule-like
  • Opioid-binding cell adhesion molecule
  • opioid-binding protein/cell adhesion molecule

Background

OBCAM (Opioid-binding cell adhesion molecule, also known as OPCML) is a member of the IgLON family of cell adhesion molecules. All IgLONs are GPI-linked glycoproteins, contain three C2 type Ig-like domains, and are expressed in cerebral cortex and hippocampus (1, 2). The name IgLON derives from family membership in the Ig superfamily, and the first letters of the names of group’s molecules; LAMP, OBCAM, and Neurotrimin. Recently, membership in the group has been expanded by one with the addition of Kilon (Kindred of IgLON), and members of this group are now often referred to Diglons, based on the dimerizing nature of the IgLONs (1, 2). Human OBCAM is synthesized as a 345 amino acid (aa) preproprecursor that contains a 27 aa signal sequence, a 295 aa mature region, and a C‑terminal 23 aa prosegment (3). The prosegment is cleaved to generate the GPI-link. OBCAM varies in molecular weight, ranging from 46 kDa to 65 kDa (4‑6). The difference is not due to alternate splicing but to differential glycosylation (6). Although it is not unusual for GPI-linked proteins to be solubilized, to date there is no evidence that OBCAM functions as a soluble molecule (1). Mature human OBCAM is 98%, 99%, and 98% aa identical to mature bovine, rat and mouse OBCAM, respectively. OBCAM has limited expression, occurring principally in telencephalon and ovarian epithelium (7, 8). In brain, it is found associated with dendrites and post-synaptic membranes, where it may maintain synaptic architecture (1, 5). In ovary, it has been suggested to be a tumor-suppressor factor (8). The receptor(s) for OBCAM appears to be other members of the IgLON family, and a dimer is the functional unit. While neurotrimin appears to function as both a homodimer and heterodimer, all other family members (including OBCAM) show a preference for heterodimerization. OBCAM forms strong trans (between cells) heterodimers with LAMP, and modest heterodimers with Neurotrimin. There is hardly any binding with itself. Kilon likely binds OBCAM, but this interaction is not well studied (1). OBCAM heterodimers apparently bind to almost all possible IgLON heterodimer combinations on other cells. In cis (same cell), OBCAM also binds to LAMP and Neurotrimin (2).

  1. Miyata, S. et al. (2000) Neuroscience 117:645.
  2. Reed, J. et al. (2004) J. Cell Sci. 117:3961.
  3. Shark, K.B. and N.M. Lee (1995) Gene 155:213.
  4. Wick, M.J. et al. (1996) Mol. Brain Res. 36:322.
  5. Miyata, S. (2000) J. Comp. Neurol. 242:74.
  6. Hachisuka, A. et al. (1996) Neurochem. Int. 28:373.
  7. Miyata, S. et al. (2003) Brain Res. 979:129.
  8. Sellar, G.C. et al. (2003) Nat. Genet. 34:337.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OBCAM/OPCML Antibody (AF2777)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol OPCML
Uniprot