DBC1/p30 Antibody - BSA Free

Immunohistochemistry: DBC1/p30 Antibody [NBP3-38390] - Immunohistochemistry analysis of paraffin-embedded Rat brain using DBC1/p30 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western Blot: DBC1/p30 Antibody [NBP3-38390] - Western blot analysis of various lysates using DBC1/p30 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Immunohistochemistry: DBC1/p30 Antibody [NBP3-38390] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using DBC1/p30 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry: DBC1/p30 Antibody [NBP3-38390] - Immunohistochemistry analysis of paraffin-embedded Human rectal cancer using DBC1/p30 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DBC1/p30 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DBC1/p30 Antibody - BSA Free (NBP3-38390) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 654-923 of human DBC1/p30 (NP_066997.3).
  
  Sequence:
  LLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGLDGGLPEEVLFGNLDLLPPPGKSTKPGAAPTEHKALVSHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRVRLAEAEETARTAERQKSQLQRLLQELRRRLTPLQLEIQRVVEKADSWVEKEEPAPSN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCAR2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DBC1/p30 Antibody - BSA Free

• DBC.1
• DBC1deleted in breast cancer 1
• DBC-1p30 DBC protein
• Deleted in breast cancer gene 1 protein
• KIAA1967
• NET35
• p30 DBC
• p30DBC

Background

DBC1 inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediatedapoptosis. Inhibits SUV39H1 methyltransferase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCAR2