ZNF326 Antibody

Western Blot: ZNF326 Antibody [NBP1-03343] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-03343 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.

Immunohistochemistry-Paraffin: ZNF326 Antibody [NBP1-03343] - Sample: FFPE sections of human ovarian carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- ZNF326 used at a dilution of 1:1,000 (0.2ug/ml) and 1:400 (0.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ZNF326 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 532 and 582 of human zinc finger protein 326 using the numbering given in entry NP_892021.1 (GeneID 284695).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF326
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:1000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:5000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZNF326 Antibody

• zinc finger protein 394
• zinc finger protein 99
• Zinc finger protein with KRAB and SCAN domains 14
• ZKSCAN14FLJ12298

Background

ZNF326 Probable transcriptional activator which may play a role in neuronal differentiation. Able to bind DNA and activate expression in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF326
• Entrez:
  • 284695(Human)
• Uniprot:
  • Q5BKZ1(Human)