DACH1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: DACH1 Antibody [NBP1-85320] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

Immunohistochemistry-Paraffin: DACH1 Antibody [NBP1-85320] - Staining of human small intestine shows strong nuclear positivity in glandular cells.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DACH1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTGLTAAAAAAAAATNAAIAEAMKVKKIKLEAMSNYHASNNQHGADSENGDMNSSVGSSDGSWDKETLPSSPSQGPQASITHPRMPGARSLPLSHPLNHLQQSHLLPNGLELP
• Predicted Species: Mouse (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DACH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  DACH1 Protein (NBP1-85320PEP)

Reactivity Notes

Rat (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DACH1 Antibody - BSA Free

• Dach1
• DACHdachshund homolog 1
• dachshund homolog (Drosophila)
• dachshund homolog 1 (Drosophila)
• FLJ10138

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DACH1 Antibody (NBP1-85320)(4)

Publications using NBP1-85320

• Zhou J, Shaikh LH, Neogi SG et al. DACH1, a Zona Glomerulosa Selective Gene in the Human Adrenal, Activates Transforming Growth Factor--beta Signaling and Suppresses Aldosterone Secretion. Hypertension 2015-05-01 [PMID: 25776071] (IF/IHC, Human)
• Wen Y, Jin S. Production of neural stem cells from human pluripotent stem cells J. Biotechnol. 2014-08-20 [PMID: 25150215]
• Vonlanthen J, Okoniewski MJ, Menigatti M et al. A comprehensive look at transcription factor gene expression changes in colorectal adenomas. BMC Cancer 2014-01-29 [PMID: 24472434] (IF/IHC, Human)
• Powe DG, Dhondalay GK, Lemetre C et al. DACH1: Its Role as a Classifier of Long Term Good Prognosis in Luminal Breast Cancer. PLoS One 2014-01-01 [PMID: 24392136]

Bioinformatics

• Gene Symbol: DACH1