SnoN Antibody

Images

 
Western Blot: SnoN Antibody [NBP1-77306] - Analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry-Paraffin: SnoN Antibody [NBP1-77306] - Mouse lung tissue with SnoN antibody at 5 ug/ml.
Immunofluorescence: SnoN Antibody [NBP1-77306] - Immunofluorescence of SnoN in Mouse Lung cells with SnoN antibody at 20 ug/mL.
Immunofluorescence: SnoN Antibody [NBP1-77306] - HeLa cells stained with SnoN antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SnoN Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SnoN. The immunogen is located within the first 50 amino acids of SnoN.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SKIL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunofluorescence 20 ug/ml
Application Notes
This antibody can be used for detection of SnoN by Western blot at 0.5 - 1 mg/ml.
Control Peptide
SnoN Peptide (NBP1-77306PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77306 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SnoN Antibody

  • SKI-like oncogene
  • ski-related oncogene snoN
  • Ski-related oncogene
  • Ski-related protein
  • SnoA
  • SnoI
  • SnoN
  • SNOski-like protein

Background

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN; SnoN antibody will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SnoN Antibody (NBP1-77306) (0)

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Review for SnoN Antibody (NBP1-77306) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC-P.

Reviews using NBP1-77306:
Filter by Applications
IHC-P
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin SnoN NBP1-77306
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reviewed by:
Anonymous
IHC-P Human 10/02/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHela cell line
SpeciesHuman

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Secondary Antibodies

 

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Recent Reviews

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1
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Anonymous
10/02/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SKIL
Uniprot