DACH2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
DACH2 was detected in perfusion fixed frozen sections of mouse embryonic brain (13 d.p.c.) using Goat Anti-Human/Mouse DACH2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5230) at 5 µg/mL for 1 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

DACH2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human DACH2
Gln419-Gln583
Accession # Q96NX9
Specificity
Detects human and mouse DACH2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DACH2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DACH2 Antibody [Unconjugated]

  • DACH2
  • dachshund homolog 2 (Drosophila)
  • dachshund homolog 2
  • FLJ31391
  • MGC138545

Background

DACH2 (Dachshund 2) is a 65 kDa (predicted) member of the DACH family of transcription factors. It is expressed in the dermomyotome and Mullerian duct, and promotes Mullerian duct formation and myogenin gene suppression. Human DACH2 is 599 amino acids (aa) in length and contains a DD1 domain in the N-terminus (aa 66‑162) that mediates DNA binding, and a C-terminal, coiled-coil DD2 domain (aa 452‑543) that interacts with EYA proteins. Multiple splice variants of DACH2 are known. There is an alternate start site at Met220 that may be accompanied by both a deletion of aa 259‑311 and a three aa substitution for aa 585‑599. In addition, there is a deletion of aa 163‑175 that may be accompanied by an Ala substitution for aa 584‑599. Finally, there is a 41 aa substitution for aa 1‑175. Over aa 419‑583, human DACH2 is 93% aa identical to mouse DACH2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-27192
Species: Hu, Mu, Bv, Ca, Ch, Eq, Pm
Applications: WB, IHC-P
NB100-56510
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
MAB2457
Species: Hu, Mu
Applications: WB, CyTOF-ready, ICC, ICFlow
NBP1-84264
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP2-22152
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-03993
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NB100-56511
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
NBP2-22151
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-61674
Species: Hu
Applications: WB, ELISA
AF1555
Species: Hu
Applications: WB
AF1638
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
NBP2-55646
Species: Hu
Applications: ICC/IF
NBP2-67471
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-93938
Species: Hu
Applications: IHC, IHC-P
NB100-58824
Species: Hu, Mu
Applications: WB, ChIP, IP, CHIP-SEQ
NBP2-01770
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-85217
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB300-750
Species: Hu, Mu, Rt, Mk, Pm, Sh
Applications: WB, ICC/IF, IP, ICC
NBP1-77305
Species: Hu
Applications: WB, ELISA, ICC/IF, IF
AF5230
Species: Hu, Mu
Applications: WB, IHC

Publications for DACH2 Antibody (AF5230) (0)

There are no publications for DACH2 Antibody (AF5230).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DACH2 Antibody (AF5230) (0)

There are no reviews for DACH2 Antibody (AF5230). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DACH2 Antibody (AF5230) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DACH2 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol DACH2
Uniprot