DCAMKL2 Antibody

Images

 
Western Blot: DCAMKL2 Antibody [NBP1-77128] - Analysis of DCLK2 in rat brain tissue lysate with DCLK2 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: DCAMKL2 Antibody [NBP1-77128] - Rat brain tissue with DCAMKL2 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DCAMKL2 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human DCLK2. The immunogen is located within the last 50 amino acids of DCLK2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DCLK2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence
Application Notes
DCLK2 antibody can be used for detection of DCLK2 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DCAMKL2 Peptide (NBP1-77128PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DCAMKL2 Antibody

  • CL2EC 2.7.11.1
  • CLICK-II
  • CLIK2
  • DCDC3
  • DCDC3BCaMK-like CREB regulatory kinase 2
  • DCK2DCAMKL2
  • DKFZp761I032
  • doublecortin and CaM kinase-like 2
  • Doublecortin domain-containing protein 3B
  • Doublecortin-like and CAM kinase-like 2
  • doublecortin-like kinase 2CLICK2
  • EC 2.7.11
  • MGC45428
  • serine/threonine-protein kinase DCLK2

Background

DCLK2 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK2 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DCLK2
Uniprot