SKI Antibody

Western Blot: SKI Antibody [NBP1-77305] - Analysis in HeLa cell lysate with antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/Immunofluorescence: SKI Antibody [NBP1-77305] - Ski in human kidney tissue with Ski antibody at 20 ug/ml.

• Reactivity: Hu, Ch, Eq
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SKI Antibody Summary

• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski. The immunogen is located within the first 50 amino acids of Ski. Amino Acid Squence: CFQPHPGLQKTLEQ
• Predicted Species: Chicken (100%), Equine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SKI
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Antibody validated: Western Blot in human samples and Immunofluorescence in human samples.
• Control Peptide:

  SKI Peptide (NBP1-77305PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Purity: Peptide affinity purified

Alternate Names for SKI Antibody

• C-ski
• Proto-oncogene c-Ski
• ski oncogene
• ski oncoprotein
• SKI
• SKV
• v-ski avian sarcoma viral oncogene homolog
• v-ski sarcoma viral oncogene homolog (avian)

Background

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SKI
• Uniprot:
  • P12755()