SKI Antibody

Images

 
Western Blot: SKI Antibody [NBP1-77305] - Analysis in HeLa cell lysate with antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: SKI Antibody [NBP1-77305] - Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 ug/ml.
Western Blot: SKI Antibody [NBP1-77305] - Analysis of SKI in human kidney tissue lysate with SKI antibody at (A) 1 and (B) 2 ug/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SKI Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski. The immunogen is located within the first 50 amino acids of Ski.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SKI
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunofluorescence
Application Notes
This antibody can be used for detection of Ski by Western blot at 1 - 2 mg/ml.
Control Peptide
SKI Peptide (NBP1-77305PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SKI Antibody

  • C-ski
  • Proto-oncogene c-Ski
  • ski oncogene
  • ski oncoprotein
  • SKV
  • v-ski avian sarcoma viral oncogene homolog
  • v-ski sarcoma viral oncogene homolog (avian)

Background

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SKI Antibody (NBP1-77305)

Discover related pathways, diseases and genes to SKI Antibody (NBP1-77305). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on SKI.

Winter Protein Games
Get into the Winter Protein Games 2014! Check out the contenders competing including SKI, POLE, ICEBURG, SKT, TRAIL, WIN and BOB1. Learn more about each protein's function, gene name, molecular weight and family.View all the fun and games happening...  Read full blog post.

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Bioinformatics

Gene Symbol SKI
Uniprot