Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free [NBP2-47983] - Human Ovarian Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (KRT8/803 + KRT18/835).

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free [NBP2-47983] - Human Colon Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (KRT8/803 + KRT18/835).

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free [NBP2-47983] - Human Lung Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (KRT8/803 + KRT18/835).

Simple Western: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free [NBP2-47983] - Simple Western lane view shows a specific band for Cytokeratin 8/18 in 0.2 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free [NBP2-47983] - Simple Western lane view shows a specific band for Cytokeratin 8/18 in 0.2 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free [NBP2-47983] - Electropherogram image of the corresponding Simple western lane. Cytokeratin 8/18 antibody was used at 10 ug/ml dilution on A431 lysate(s) respectively.

Simple Western: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free [NBP2-47983] - Lane view shows a specific band for Cytokeratin 8/18. 200 ug/mL of A431 cell lysate and 50 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 kDa. Image from an internal validation.

• Reactivity: Hu
• Applications: Simple Western, Flow, IHC, CyTOF-ready
• Clone: KRT8/803 + KRT18/835
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44930).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human KRT8 protein (KRT8/803); Recombinant full-length human KRT18 protein (KRT18/835)
• Localization: Cytoplasmic
• Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This monoclonal antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immuno-histochemical staining with this monoclonal antibody is indistinguishable from that obtained with monoclonal antibody 5D3.
• Isotype: IgG1 Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT8
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Simple Western 20 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: antibody dilution of 20 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free

• CARD2
• CK8
• CK-8
• CYK8cytokeratin 8
• Cytokeratin-8
• K2C8
• K8cytokeratin-8
• keratin 8
• keratin, type II cytoskeletal 8
• keratin-8
• KO
• Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT8
• Uniprot:
  • P05783(Human)
  • P05787(Human)