Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835)

Images

 
Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) [NBP2-44930] - Human Ovarian Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (KRT8/803 + KRT18/835).
Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) [NBP2-44930] - Human Colon Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (KRT8/803 + KRT18/835).
Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) [NBP2-44930] - Human Lung Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (KRT8/803 + KRT18/835).
Simple Western: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) [NBP2-44930] - Simple Western lane view shows a specific band for Cytokeratin 8/18 in 0.2 mg/ml of A431 lysate. This experiment was performed under ...read more
Simple Western: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) [NBP2-44930] - Simple Western lane view shows a specific band for Cytokeratin 8/18 in 0.2 mg/ml of A431 lysate. This experiment was performed under ...read more
Simple Western: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) [NBP2-44930] - Electropherogram image of the corresponding Simple western lane. Cytokeratin 8/18 antibody was used at 10 ug/ml dilution on A431 lysate(s) ...read more
Simple Western: Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) [NBP2-44930] - Electropherogram image of the corresponding Simple western lane view. Cytokeratin 8/18 antibody was used at 10 ug/ml dilution on MCF-7 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Simple Western, Flow, IHC, IHC-P
Clone
KRT8/803 + KRT18/835
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) Summary

Immunogen
Recombinant full-length human KRT8 protein (KRT8/803); Recombinant full-length human KRT18 protein (KRT18/835)
Localization
Cytoplasmic
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This monoclonal antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immuno-histochemical staining with this monoclonal antibody is indistinguishable from that obtained with monoclonal antibody 5D3.
Isotype
IgG1 Kappa/IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.1-0.2 ug/ml
  • Simple Western 10 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47983)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835)

  • CARD2
  • CK8
  • CK-8
  • CYK8cytokeratin 8
  • Cytokeratin-8
  • K2C8
  • K8cytokeratin-8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KRT8
Uniprot