Cytokeratin 5 Antibody (EP1601Y)

Western Blot: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Expression of cytokeratins (CK) and GAPDH in HMGEC. Representative western blots show specific bands for GAPDH, CK1, Cytokeratin 5, CK6 and CK14. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0128096) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - A431 cells.

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 (EP1601Y) on prostate.

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 antibody labels squamous elements in the basal epidermis and sebaceous glands.

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Rabbit monoclonal CK5 antibody strongly highlights mesothelioma cells in a cytoplasmic pattern.

Simple Western: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Simple Western lane view shows a specific band for Cytokeratin 5 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: EP1601Y
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.

Cytokeratin 5 Antibody (EP1601Y) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human CK-5 was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT5
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-250
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Simple Western 1:100
  • Western Blot
• Application Notes: Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26419726) This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cytokeratin 5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Culture supernatant in buffered aqueous solution
• Preservative: No Preservative
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Tissue culture supernatant

Alternate Names for Cytokeratin 5 Antibody (EP1601Y)

• 58 kDa cytokeratin
• CK5
• CK-5
• cytokeratin-5
• DDD
• EBS2
• epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
• K5
• keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
• keratin 5
• keratin, type II cytoskeletal 5
• Keratin-5
• KRT5A
• Type-II keratin Kb5

Background

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 5 Antibody (NB110-56916)(8)

Publications using NB110-56916

• Li S, Goncalves KA, Lyu B et al. Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors Commun Biol 2020-01-15 [PMID: 31942000]
• Li Z, Zeng M, Deng Y et al. Nasal polyps 15-lipoxygenase 1 promotes CCL26/Eotaxin3 expression through ERK activation J. Allergy Clin. Immunol. 2019-07-10 [PMID: 31301373] (ICC/IF, Human)
• Gindele JA, Mang S, Pairet N et al. Opposing effects of in vitro differentiated macrophages sub-type on epithelial wound healing PLoS ONE 2017-09-01 [PMID: 28863189] (ICC/IF, Human)
• Pedrosa AR, Graça JL, Carvalho S et al. Notch signaling dynamics in the adult healthy prostate and in prostatic tumor development. Prostate 2016-01-01 [PMID: 26419726] (IHC-Fr)
• Hampel U, Schroder A, Mitchell T et al. Serum-induced keratinization processes in an immortalized human meibomian gland epithelial cell line. PLoS One 2015-01-01 [PMID: 26042605] (Human)
• Miura Y, Harumiya S, Ono K et al. Galectin-7 and actin are components of amyloid deposit of localized cutaneous amyloidosis Exp Dermatol 2013-01-01 [PMID: 23278892] (IF/IHC, Human)
• Alexander M, Reising B, Schumacher S et al. Validation of markers for the screening and identification of disseminating tumor cells in lymph nodes and bone marrow. Int J Clin Pharmacol Ther 2013-01-01 [PMID: 23260007] (ICC/IF, Human)
• Mochizuki Y, Omura K, Sakamoto K et al. A case of primary combined neuroendocrine carcinoma with squamous cell carcinoma in the upper gingiva. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010-04-01 [PMID: 20303044]

Bioinformatics

• Gene Symbol: KRT5
• Entrez:
  • 3852(Human)
  • 110308(Mouse)
  • 369017(Rat)
• Uniprot:
  • P13647()