Cytokeratin 5 Antibody (EP1601Y)

Images

 
Biological Strategies: Western Blot: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Expression of cytokeratins (CK) and GAPDH in HMGEC. Representative western blots show specific bands for GAPDH, CK1, ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - A431 cells.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 (EP1601Y) on prostate.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 antibody labels squamous elements in the basal epidermis and sebaceous glands.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Rabbit monoclonal CK5 antibody strongly highlights mesothelioma cells in a cytoplasmic pattern.
Simple Western: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Simple Western lane view shows a specific band for Cytokeratin 5 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
EP1601Y
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Validated by:
   

Biological Strategies

     

Order Details

Cytokeratin 5 Antibody (EP1601Y) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
A synthetic peptide corresponding to residues on the C-terminus of human CK-5 was used as an immunogen.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
KRT5
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:100-250
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26419726) This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Cytokeratin 5 Overexpression Lysate
Publications
Read Publications using
NB110-56916 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Culture supernatant in buffered aqueous solution
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Tissue culture supernatant

Alternate Names for Cytokeratin 5 Antibody (EP1601Y)

  • 58 kDa cytokeratin
  • CK5
  • CK-5
  • cytokeratin-5
  • DDD
  • EBS2
  • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
  • K5
  • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
  • keratin 5
  • keratin, type II cytoskeletal 5
  • Keratin-5
  • KRT5A
  • Type-II keratin Kb5

Background

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-56916
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Cytokeratin 5 Antibody (NB110-56916)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-Fr.


Filter By Application
ICC/IF
(3)
IHC
(1)
IHC-Fr
(1)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB110-56916 Applications Species
Li S, Goncalves KA, Lyu B et al. Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors Commun Biol Jan 15 2020 [PMID: 31942000]
Li Z, Zeng M, Deng Y et al. Nasal polyps 15-lipoxygenase 1 promotes CCL26/Eotaxin3 expression through ERK activation J. Allergy Clin. Immunol. Jul 10 2019 [PMID: 31301373] (ICC/IF, Human) ICC/IF Human
Gindele JA, Mang S, Pairet N et al. Opposing effects of in vitro differentiated macrophages sub-type on epithelial wound healing PLoS ONE 2017 Sep 01 [PMID: 28863189] (ICC/IF, Human) ICC/IF Human
Pedrosa AR, Graça JL, Carvalho S et al. Notch signaling dynamics in the adult healthy prostate and in prostatic tumor development. Prostate 2016 Jan [PMID: 26419726] (IHC-Fr) IHC-Fr
Hampel U, Schroder A, Mitchell T et al. Serum-induced keratinization processes in an immortalized human meibomian gland epithelial cell line. PLoS One 2015 [PMID: 26042605] (Human) Human
Miura Y, Harumiya S, Ono K et al. Galectin-7 and actin are components of amyloid deposit of localized cutaneous amyloidosis Exp Dermatol 2013 Jan [PMID: 23278892] (IHC, Human) IHC Human
Alexander M, Reising B, Schumacher S et al. Validation of markers for the screening and identification of disseminating tumor cells in lymph nodes and bone marrow. Int J Clin Pharmacol Ther 2013 Jan [PMID: 23260007] (ICC/IF, Human) ICC/IF Human
Mochizuki Y, Omura K, Sakamoto K et al. A case of primary combined neuroendocrine carcinoma with squamous cell carcinoma in the upper gingiva. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Apr. [PMID: 20303044]

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Product General Protocols

View specific protocols for Cytokeratin 5 Antibody (NB110-56916): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT5
Entrez
Uniprot