Cytokeratin 5 Antibody (EP1601Y)


Biological Strategies: Western Blot: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Expression of cytokeratins (CK) and GAPDH in HMGEC. Representative western blots show specific bands for GAPDH, CK1, more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - A431 cells.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 (EP1601Y) on prostate.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 antibody labels squamous elements in the basal epidermis and sebaceous glands.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Rabbit monoclonal CK5 antibody strongly highlights mesothelioma cells in a cytoplasmic pattern.
Simple Western: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Simple Western lane view shows a specific band for Cytokeratin 5 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions more

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Cytokeratin 5 Antibody (EP1601Y) Summary

Additional Information
Recombinant Monoclonal Antibody.
A synthetic peptide corresponding to residues on the C-terminus of human CK-5 was used as an immunogen.
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Simple Western 1:100
  • Western Blot
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26419726) This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Cytokeratin 5 Overexpression Lysate
Read Publications using
NB110-56916 in the following applications:

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Culture supernatant in buffered aqueous solution
No Preservative
This product is unpurified. The exact concentration of antibody is not quantifiable.
Tissue culture supernatant

Alternate Names for Cytokeratin 5 Antibody (EP1601Y)

  • 58 kDa cytokeratin
  • CK5
  • CK-5
  • cytokeratin-5
  • DDD
  • EBS2
  • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
  • K5
  • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
  • keratin 5
  • keratin, type II cytoskeletal 5
  • Keratin-5
  • KRT5A
  • Type-II keratin Kb5


Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 5 Antibody (NB110-56916)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC-Fr.

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Showing Publications 1 - 8 of 8.
Publications using NB110-56916 Applications Species
Li S, Goncalves KA, Lyu B et al. Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors Commun Biol 2020-01-15 [PMID: 31942000]
Li Z, Zeng M, Deng Y et al. Nasal polyps 15-lipoxygenase 1 promotes CCL26/Eotaxin3 expression through ERK activation J. Allergy Clin. Immunol. 2019-07-10 [PMID: 31301373] (ICC/IF, Human) ICC/IF Human
Gindele JA, Mang S, Pairet N et al. Opposing effects of in vitro differentiated macrophages sub-type on epithelial wound healing PLoS ONE 2017-09-01 [PMID: 28863189] (ICC/IF, Human) ICC/IF Human
Pedrosa AR, Graça JL, Carvalho S et al. Notch signaling dynamics in the adult healthy prostate and in prostatic tumor development. Prostate 2016-01-01 [PMID: 26419726] (IHC-Fr) IHC-Fr
Hampel U, Schroder A, Mitchell T et al. Serum-induced keratinization processes in an immortalized human meibomian gland epithelial cell line. PLoS One 2015-01-01 [PMID: 26042605] (Human) Human
Miura Y, Harumiya S, Ono K et al. Galectin-7 and actin are components of amyloid deposit of localized cutaneous amyloidosis Exp Dermatol 2013-01-01 [PMID: 23278892] (IF/IHC, Human) IF/IHC Human
Alexander M, Reising B, Schumacher S et al. Validation of markers for the screening and identification of disseminating tumor cells in lymph nodes and bone marrow. Int J Clin Pharmacol Ther 2013-01-01 [PMID: 23260007] (ICC/IF, Human) ICC/IF Human
Mochizuki Y, Omura K, Sakamoto K et al. A case of primary combined neuroendocrine carcinoma with squamous cell carcinoma in the upper gingiva. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010-04-01 [PMID: 20303044]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

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Gene Symbol KRT5