Cytokeratin 5 Antibody (EP1601Y)

Images

 
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - A431 cells.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 (EP1601Y) on prostate.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 antibody labels squamous elements in the basal epidermis and sebaceous glands.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Rabbit monoclonal CK5 antibody strongly highlights mesothelioma cells in a cytoplasmic pattern.
Simple Western: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Simple Western lane view shows a specific band for Cytokeratin 5 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
EP1601Y
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Cytokeratin 5 Antibody (EP1601Y) Summary

Immunogen
A synthetic peptide corresponding to residues on the C-terminus of human CK-5 was used as an immunogen.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
KRT5
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:100-250
  • Immunohistochemistry 1:100-250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-250
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26419726) This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Cytokeratin 5 Lysate (NBL1-12393)
Publications
Read Publications using
NB110-56916 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Culture supernatant in buffered aqueous solution
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Tissue culture supernatant

Alternate Names for Cytokeratin 5 Antibody (EP1601Y)

  • 58 kDa cytokeratin
  • CK5
  • CK-5
  • cytokeratin-5
  • DDD
  • EBS2
  • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
  • K5
  • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
  • keratin 5
  • keratin, type II cytoskeletal 5
  • Keratin-5
  • KRT5A
  • Type-II keratin Kb5

Background

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34270
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NBP2-45677
Species: Hu, Mk
Applications: WB, IHC
NBP2-33090
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP1-26642
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
AF5415
Species: Hu
Applications: WB, IHC, ICC
NBP2-29429
Species: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, Flow-IC, Single Cell Western
NB100-355
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Xp
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, ICC
NBP2-24482
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: WB
NBP2-16094
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88080
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP2-61736
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, IHC
NB100-687
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-85630
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-85632
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-104
Species: Hu, Mu
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, ICC/IF, ICC
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB110-56916
Species: Hu
Applications: Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Cytokeratin 5 Antibody (NB110-56916)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-Fr.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-Fr
(1)
All Applications
Filter By Species
Human
(4)
All Species

Reviews for Cytokeratin 5 Antibody (NB110-56916) (0)

There are no reviews for Cytokeratin 5 Antibody (NB110-56916). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for Cytokeratin 5 Antibody (NB110-56916): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Cytokeratin 5 Antibody (NB110-56916) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Cytokeratin 5 Antibody (NB110-56916)

Discover related pathways, diseases and genes to Cytokeratin 5 Antibody (NB110-56916). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cytokeratin 5 Antibody (NB110-56916)

Discover more about diseases related to Cytokeratin 5 Antibody (NB110-56916).
 

Pathways for Cytokeratin 5 Antibody (NB110-56916)

View related products by pathway.

PTMs for Cytokeratin 5 Antibody (NB110-56916)

Learn more about PTMs related to Cytokeratin 5 Antibody (NB110-56916).
 

Research Areas for Cytokeratin 5 Antibody (NB110-56916)

Find related products by research area.

Blogs on Cytokeratin 5

There are no specific blogs for Cytokeratin 5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cytokeratin 5 Antibody (EP1601Y) and receive a gift card or discount.

Bioinformatics

Gene Symbol KRT5
Entrez
Uniprot