Biological Strategies: Western Blot: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Expression of cytokeratins (CK) and GAPDH in HMGEC. Representative western blots show specific bands for GAPDH, CK1, ...read more
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 antibody labels squamous elements in the basal epidermis and sebaceous glands.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Rabbit monoclonal CK5 antibody strongly highlights mesothelioma cells in a cytoplasmic pattern.
Simple Western: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Simple Western lane view shows a specific band for Cytokeratin 5 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions ...read more
WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26419726) This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
62 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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