Cytokeratin 5 Antibody (2C8E4) - BSA Free

Images

 
Western Blot: Cytokeratin 5 Antibody (2C8E4) [NBP2-52443] - Analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), SK-Br-3 (3), Hela (4), Lncap (5), and HepG2 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (2C8E4) [NBP2-52443] - Analysis of MCF-7 cells using CK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with ...read more
Immunohistochemistry: Cytokeratin 5 Antibody (2C8E4) [NBP2-52443] - Analysis of paraffin-embedded lung cancer tissues using CK5 mouse mAb with DAB staining.
Flow Cytometry: Cytokeratin 5 Antibody (2C8E4) [NBP2-52443] - Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).
Western Blot: Cytokeratin 5 Antibody (2C8E4) [NBP2-52443] - Analysis using CK5 mAb against HEK293 (1) and CK5 (AA: 258-357)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Cytokeratin 5 Antibody (2C8E4) [NBP2-52443] - Analysis using CK5 mAb against human CK5 (AA: 258-357) recombinant protein. (Expected MW is 33.1 kDa)
ELISA: Cytokeratin 5 Antibody (2C8E4) [NBP2-52443] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
2C8E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

Cytokeratin 5 Antibody (2C8E4) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CK5 (AA: 258-357) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
62.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytokeratin 5 Antibody (2C8E4) - BSA Free

  • 58 kDa cytokeratin
  • CK5
  • CK-5
  • cytokeratin-5
  • DDD
  • EBS2
  • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
  • K5
  • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
  • keratin 5
  • keratin, type II cytoskeletal 5
  • Keratin-5
  • KRT5A
  • Type-II keratin Kb5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT5