Cytokeratin 18 Antibody (810811) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cytokeratin 18 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Cytokeratin 18 at approximately 55 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Cytokeratin 18 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to intermediate filaments in cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cytokeratin 18 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clone: 810811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cytokeratin 18 Antibody (810811) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Cytokeratin 18
  Ala239-Asp397
  Accession # P05783
• Specificity: Detects human Cytokertin 18 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL
  • Simple Western 2 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.
• Control:

  Cytokeratin 18 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytokeratin 18 Antibody (810811) [Unconjugated]

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Background

Cytokeratin 18; also KRT-18 (Keratin, type I cytoskeletal 18), Cell proliferation-inducing gene 46 and Keratin-18) is a 44-48 kDa Class I (large keratins of acidic pH) member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class I Cytokeratin 18 heterodimerizes/polymerizes with 50-52 kDa class II KRT-8 to form 8-10 nm filaments in single strata plus hepatic epithelia. Cytokeratin 18 and -8 are the first keratins to appear in the mammalian embyro. In the adult, Cytokeratin 18 appears to participate in subtractions and additions to the plasma membrane. In this regard, a number of intracellular proteins interact with Cytokeratin 18, including 14-3-3, HSPc70 and Mrj. Cytokeratin 18 may also be O‑glycosylated, and when so, serves to promote Akt-1 activity, thus protecting against apoptosis. Human Cytokeratin 18 is 430 amino acids (aa) in length. It contains an N-terminal "head" region (aa 1-79), a subsequent "rod" region (aa 80-387) with two coiled segments, and a C-terminal tail region. Cytokeratin 18 possesses at least 19 utilized phosphorylation sites plus five acetylated Lys residues. There are multiple isoforms that range from 20-40 kDa in size and are the result of caspase cleavage. A principal cleavage site occurs after Asp238. Over aa 239-397, human Cytokeratin 18 shares 86% aa sequence identity with mouse Cytokeratin 18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 18 Antibody (MAB7619)(1)

Publication using MAB7619

• W Li, X Zhang, J Wang, M Li, C Cao, J Tan, D Ma, Q Gao TGF?1 in fibroblasts-derived exosomes promotes epithelial-mesenchymal transition of ovarian cancer cells Oncotarget, 2017;8(56):96035-96047. 2017 [PMID: 29221185] (Flow Cytometry, Human)

Review for Cytokeratin 18 Antibody (MAB7619) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous ICC Human 11/28/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: pig trabecular meshwork cells
• Species: Human
• Lot: N/A

Bioinformatics

• Gene Symbol: KRT18
• Uniprot:
  • P05783(Human)