Cytokeratin 18 Antibody (DE-K18)

Images

 
Western Blot: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DE-K18).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Confocal Immunofluorescence image of HeLa cells using Cytokeratin 18 Antibody (DE-K18) Green (CF488) and Red Dot is used to label ...read more
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (DE-K18).
Flow Cytometry: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Flow Cytometric Analysis of trypsinised MeOH-fixed HeLa cells using Cytokeratin 18 Antibody (DE-K18) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype ...read more
Western Blot: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Western Blot Analysis of MCF-7cell lysate using Cytokeratin 18 Antibody (DE-K18).
Simple Western: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions ...read more
Simple Western: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.

Product Details

Summary
Reactivity Hu, Ca, Fe, Rt(-)Species Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Clone
DE-K18
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Catalog# & Formulation Size Price

Cytokeratin 18 Antibody (DE-K18) Summary

Immunogen
Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431
Localization
Cytoplasmic
Marker
Epithelial Marker
Specificity
This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT18
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Simple Western 10 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11516)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 18 Antibody (DE-K18)

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • Cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Cytokeratin 18 Antibody (NBP2-44947)

Discover related pathways, diseases and genes to Cytokeratin 18 Antibody (NBP2-44947). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Cytokeratin 18.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT18
Uniprot