Cytokeratin 18 Antibody (DC10)

Images

 
Western Blot: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Analysis using the Azide and BSA Free version of NBP2-29461. Detection of Keratin 18 in 1) HeLa and 2) A431 lysate using Keratin 18 antibody at 1 ug/ml. goat ...read more
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Analysis using the Azide and BSA Free version of NBP2-29461. Staining of human skin sweat gland. Note cytoplasmic staining of tumor cells.
Simple Western: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions ...read more
Simple Western: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.

Product Details

Summary
Reactivity Hu, Bv(-), Ca(-), Ha(-), Mu(-), Po(-), Rt(-), Sh(-)Species Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
DC10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Cytokeratin 18 Antibody (DC10) Summary

Immunogen
Human breast cancer PMC 42 cells were used as immunogen to generate the Keratin 18 antibody.
Localization
Cytoplasmic
Marker
Epithelial Marker
Specificity
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT18
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3ug/ml
  • Simple Western 10 ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein
Application Notes
Use in Immunohistochemistry-Frozen has been reported in literature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-29461.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for Cytokeratin 18 Antibody (DC10)

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • Cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18

Background

Keratins, also called cytokeratins, comprise a family of filamentous structural proteins that form the intermediate filaments of epithelial cells. Keratin proteins are differentially expressed depending on epithelial cell type and degree of differentiation. Antibody to a given keratin can be useful as a marker in of itself or as part of an antibody panel to help identify or classify tissue origin.

The DC10 antibody clone reacts with Keratin 18, a 45 kDa molecular weight polypeptide that is expressed in combination with Keratin 8. Keratin 18 and 8 are the most commonly expressed intermediate filaments in single layer or simple epithelial tissues. The antibody recognizes a wide variety of simple epithelia, including glandular epithelium, but not stratified squamous epithelia (Lauerova et al, 1988).

Keratin 18 is often persistently expressed in tumor cells derived from simple epithelia, and the DC10 antibody has been useful in studies for defining tissue origin (Lane et al, 1990, Kovarik et al, 1988). For example, a cheek mass was found to be negative for the keratin 18 antibody, helping to rule out an epithelial cancer (Terada, 2011). The antibody has also been used define keratin expression patterns and develop markers for tumor subtyping. For example, antibody staining results suggested that differential expression of keratin 18 in jaw tumors may useful in distinguishing ameloblastic carcinomas (keratin 18 positive) from ameloblastomas (keratin 18 negative).

The antibody is widely published; researchers are encouraged to perform a key word search on scholar.google.com for additional information about how it has been used.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-16094
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-56905
Species: Hu
Applications: WB, ICC/IF
NBP1-78278
Species: Hu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, ICC/IF, ICC
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-34355
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-88080
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
MAB1368
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow, KO
AF3844
Species: Hu, Mu
Applications: IHC
NBP2-22194
Species: Hu, Mu, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
294-HG/CF
Species: Hu
7270-IL/CF
Species: Hu
MAB25031
Species: Hu
Applications: WB, IHC, IP
NBP1-85632
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-29461
Species: Hu, Bv(-), Ca(-), Ha(-), Mu(-), Po(-), Rt(-), Sh(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for Cytokeratin 18 Antibody (NBP2-29461)(2)

Reviews for Cytokeratin 18 Antibody (NBP2-29461) (0)

There are no reviews for Cytokeratin 18 Antibody (NBP2-29461). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 18 Antibody (NBP2-29461) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Cytokeratin 18 Antibody (NBP2-29461)

Discover related pathways, diseases and genes to Cytokeratin 18 Antibody (NBP2-29461). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cytokeratin 18 Antibody (NBP2-29461)

Discover more about diseases related to Cytokeratin 18 Antibody (NBP2-29461).
 

Pathways for Cytokeratin 18 Antibody (NBP2-29461)

View related products by pathway.

PTMs for Cytokeratin 18 Antibody (NBP2-29461)

Learn more about PTMs related to Cytokeratin 18 Antibody (NBP2-29461).
 

Research Areas for Cytokeratin 18 Antibody (NBP2-29461)

Find related products by research area.

Blogs on Cytokeratin 18.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cytokeratin 18 Antibody (DC10) and receive a gift card or discount.

Bioinformatics

Gene Symbol KRT18
Uniprot