Cytokeratin 18 Antibody (DC10)


Western Blot: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Analysis using the Azide and BSA Free version of NBP2-29461. Detection of Keratin 18 in 1) HeLa and 2) A431 lysate using Keratin 18 antibody at 1 ug/ml. goat more
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Analysis using the Azide and BSA Free version of NBP2-29461. Staining of human skin sweat gland. Note cytoplasmic staining of tumor cells.
Simple Western: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions more
Simple Western: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.

Product Details

Reactivity Hu, Bv(-), Ca(-), Ha(-), Mu(-), Po(-), Rt(-), Sh(-)Species Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
0.2 mg/ml

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Cytokeratin 18 Antibody (DC10) Summary

Human breast cancer PMC 42 cells were used as immunogen to generate the Keratin 18 antibody.
Epithelial Marker
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
Protein G purified
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  • Western Blot 1-3ug/ml
  • Simple Western 10 ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein
Application Notes
Use in Immunohistochemistry-Frozen has been reported in literature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP2-29461.

Packaging, Storage & Formulations

Store at 4C.
PBS with 0.05% BSA.
0.05% Sodium Azide
0.2 mg/ml
Protein G purified

Alternate Names for Cytokeratin 18 Antibody (DC10)

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • Cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18


Keratins, also called cytokeratins, comprise a family of filamentous structural proteins that form the intermediate filaments of epithelial cells. Keratin proteins are differentially expressed depending on epithelial cell type and degree of differentiation. Antibody to a given keratin can be useful as a marker in of itself or as part of an antibody panel to help identify or classify tissue origin.

The DC10 antibody clone reacts with Keratin 18, a 45 kDa molecular weight polypeptide that is expressed in combination with Keratin 8. Keratin 18 and 8 are the most commonly expressed intermediate filaments in single layer or simple epithelial tissues. The antibody recognizes a wide variety of simple epithelia, including glandular epithelium, but not stratified squamous epithelia (Lauerova et al, 1988).

Keratin 18 is often persistently expressed in tumor cells derived from simple epithelia, and the DC10 antibody has been useful in studies for defining tissue origin (Lane et al, 1990, Kovarik et al, 1988). For example, a cheek mass was found to be negative for the keratin 18 antibody, helping to rule out an epithelial cancer (Terada, 2011). The antibody has also been used define keratin expression patterns and develop markers for tumor subtyping. For example, antibody staining results suggested that differential expression of keratin 18 in jaw tumors may useful in distinguishing ameloblastic carcinomas (keratin 18 positive) from ameloblastomas (keratin 18 negative).

The antibody is widely published; researchers are encouraged to perform a key word search on for additional information about how it has been used.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 18 Antibody (NBP2-29461)(2)

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WB Video Protocol
ICC/IF Video Protocol

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Blogs on Cytokeratin 18.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Gene Symbol KRT18