Cytokeratin 18 Antibody (DA7)

Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (DA7).

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Confocal immunofluorescence image of MCF cells using Cytokeratin 18 Antibody (DA7). Green (CF488) and Red Dot is used to label the nuclei.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Formalin-paraffin human colon cancer stained with Cytokeratin 18 Ab (DA7). Note cytoplasmic staining of tumor cells.

Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Analysis of Cytokeratin 18 in HeLa lysate using Cytokeratin 18 (CK18) antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.

• Reactivity: Hu, Bv(-), Ca(-), Ha(-), Mu(-), Po(-), Rt(-), Sh(-)
• Applications: WB, Simple Western, Flow, ICC/IF, IHC
• Clone: DA7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 18 Antibody (DA7) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33176)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human breast cancer PMC 42 cells
• Localization: Cytoplasmic
• Marker: Epithelial Marker
• Specificity: This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western 10 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 56 kDa.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Mouse, Rat, Sheep, Hamster, Bovine, Canine or Porcine.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 18 Antibody (DA7)

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Background

Keratins, also called cytokeratins, comprise a family of filamentous structural proteins that form the intermediate filaments of epithelial cells. Cytokeratin proteins are differentially expressed depending on epithelial cell type and degree of differentiation. Antibody to a given cytokeratin can be useful as a marker in of itself or as part of an antibody panel to help identify or classify tissue origin. The DA7 antibody clone reacts with Keratin 18 (CK18), a 45 kDa molecular weight polypeptide that is expressed in combination with Keratin 8. Cytokeratin 18 and 8 are the most commonly expressed intermediate filaments in single layer or simple epithelial tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT18
• Uniprot:
  • P05783(Human)