Cytokeratin 18 Antibody (Cocktail) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (Cocktail) - IHC-Prediluted [NBP2-48076] - Human Lung Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (Cocktail) - IHC-Prediluted [NBP2-48076] - Human Cervical Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (Cocktail) - IHC-Prediluted [NBP2-48076] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (Cocktail) - IHC-Prediluted [NBP2-48076] - Human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (Cocktail) - IHC-Prediluted [NBP2-48076] - Human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).

Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (Cocktail) - IHC-Prediluted.

Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 18 Antibody (Cocktail) - IHC-Prediluted followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

• Reactivity: Hu
• Applications: IHC
• Clone: Cocktail
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Cytokeratin 18 Antibody (Cocktail) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Recombinant full-length human Cytokeratin 18 protein (Uniprot: P05783)
• Localization: Cytoplasmic
• Specificity: This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
• Isotype: IgG1 Kappa/IgG1 Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 18 Antibody (Cocktail) - IHC-Prediluted

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• Keratin-18
• KRT18

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT18