Cytokeratin 14 Antibody (SC65-06)

Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat

Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/400 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Human skin

Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Product Discontinued

Cytokeratin 14 Antibody (SC65-06) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Mouse Cytokeratin 14 aa 250-484 / 484. (SwissProt: P02533 Human; SwissProt: Q61781 Mouse; SwissProt: Q6IFV1 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cytokeratin 14 Antibody (SC65-06)

• CK14
• CK-14
• Cytokeratin 14
• cytokeratin-14
• EBS3
• EBS4
• K14
• keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
• keratin 14
• keratin, type I cytoskeletal 14
• keratin-14
• KRT14
• NFJ

Background

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 14 Antibody (NBP2-67585)(2)

Publications using NBP2-67585

• Weng Y, Wang H, Wu D et al. A novel lineage of osteoprogenitor cells with dual epithelial and mesenchymal properties govern maxillofacial bone homeostasis and regeneration after MSFL Cell research Jul 12 2022 [PMID: 35821090] (IHC-P, Human, Mouse)
• Supp DM, Hahn JM, Combs KA et al. Isolation and feeder-free primary culture of four cell types from a single human skin sample STAR protocols Mar 18 2022 [PMID: 35199036]

Bioinformatics

• Gene Symbol: KRT14