Cytokeratin 14 Antibody (D19-N)

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (D19-N) [NBP1-79069] - CK14 expression in ductal myoepithelial cells of the breast. Formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti-Cytokeratin 14 monospecific clonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (D19-N) [NBP1-79069] - CK14 expression in the epidermal and skin-adnexal epithelial cells. All, formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti-Cytokeratin 14 monospecific clonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (D19-N) [NBP1-79069] - CK14 expression in the myoepithelial cells of DCIS of the breast. Formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti-Cytokeratin 14 monospecific clonal antibody.

• Reactivity: Hu
• Applications: IHC
• Clone: D19-N
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cytokeratin 14 Antibody (D19-N) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Immunogen: Peptide derived from C-terminal region of human cytokeratin 14. Antibody recognizes the epitope between Val455 - Lys471.
• Epitope: Val455 - Lys471
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
• Application Notes: Although not tested this antibody may be useful in Immunohistochemistry frozen sections as well.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for Cytokeratin 14 Antibody (D19-N)

• CK14
• CK-14
• Cytokeratin 14
• Cytokeratin-14
• EBS3
• EBS4
• K14
• keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
• keratin 14
• keratin, type I cytoskeletal 14
• Keratin-14
• KRT14
• NFJ

Background

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT14