Cytochrome P450 1A2 Antibody (D15)

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
D15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Cytochrome P450 1A2 Antibody (D15) Summary

Immunogen
MC1a (Preparation C31B4, rat liver cytochrome P4501A2)
Localization
Liver endoplasmic reticulum, membrane bound.
Specificity
D15 cross reacts with CYP1A1 & CYP1A2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYP1A2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

Positive control(s): MCF7 cells (IF) or human normal liver (IHC).

Alternate Names for Cytochrome P450 1A2 Antibody (D15)

  • aryl hydrocarbon hydroxylase
  • CP12
  • CYPIA2
  • Cytochrome P(3)450
  • cytochrome P450 1A2
  • Cytochrome P450 4
  • cytochrome P450, family 1, subfamily A, polypeptide 2
  • cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2
  • Cytochrome P450-P3
  • dioxin-inducible P3-450
  • EC 1.14.14.1
  • flavoprotein-linked monooxygenase
  • microsomal monooxygenase
  • P3-450
  • P450 form 4
  • P450(PA)
  • xenobiotic monooxygenase

Background

In animals, P450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. CYP1A2 is an aryl hydrocarbon hydroxylase involved in the metabolism of endogenous compounds and xenobiotics. It is one of the major drug metabolising enzymes in humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYP1A2
Entrez