Cytochrome P450 1A2 Antibody (D15) - BSA Free

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: D15
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Cytochrome P450 1A2 Antibody (D15) - BSA Free Summary

• Description: Novus Biologicals Mouse Cytochrome P450 1A2 Antibody (D15) - BSA Free (NB600-1490) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: MC1a (Preparation C31B4, rat liver cytochrome P4501A2)
• Localization: Liver endoplasmic reticulum, membrane bound.
• Specificity: D15 cross reacts with CYP1A1 & CYP1A2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYP1A2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s): MCF7 cells (ICC/IF) or human normal liver (IHC).
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cytochrome P450 1A2 Antibody (D15) - BSA Free

• aryl hydrocarbon hydroxylase
• CP12
• CYP1A2
• CYPIA2
• Cytochrome P(3)450
• Cytochrome P450 1A2
• Cytochrome P450 4
• cytochrome P450, family 1, subfamily A, polypeptide 2
• cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2
• Cytochrome P450-P3
• dioxin-inducible P3-450
• EC 1.14.14.1
• flavoprotein-linked monooxygenase
• microsomal monooxygenase
• P3-450
• P450 form 4
• P450(PA)
• xenobiotic monooxygenase

Background

In animals, P450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. CYP1A2 is an aryl hydrocarbon hydroxylase involved in the metabolism of endogenous compounds and xenobiotics. It is one of the major drug metabolising enzymes in humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP1A2
• Entrez:
  • 1544(Human)
  • 13077(Mouse)
  • 24297(Rat)