Cystatin A Antibody [Biotin]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Cystatin A Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Cystatin A
  Ile2-Phe98
  Accession # P01040
• Specificity: Detects human Cystatin A in Western blots. In Western blots, less than 2% cross‑reactivity with recombinant human (rh) Cystatin B, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin F, rhCystatin S, rhCystatin SA and rhCystatin SN is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSTA
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Cystatin A, also called stefin A, Cystatin AS or keratolinin, is a member of family 1 of the cystatin superfamily (1, 2). Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (3). For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes (4). The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma (5). The human Cystatin A consists of 98 amino acids (1).

1. Kartasova, T. et al. (1987) Nucleic Acids Res. 15:5945.
2. Abrahamson, M. (1994) Methods Enzymol. 244:685.
3. Jenko, S. et al. (2003) J. Mol. Biol. 326:875.
4. Palungwachira, P. et al. (2002) J. Dermatol. 29:573.
5. Sinha, A.A. et al. (2002) Cancer 94:3141.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSTA