CYP7B1 Antibody

Western Blot: CYP7B1 Antibody [H00009420-A01] - Analysis of CYP7B1 expression in NIH/3T3 (Cat # L018V1).

Western Blot: CYP7B1 Antibody [H00009420-A01] - Analysis of CYP7B1 expression in PC-12.

Product Discontinued

CYP7B1 Antibody Summary

• Immunogen: CYP7B1 (NP_004811, 203 a.a. - 286 a.a.) partial recombinant protein with GST tag. CDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHH
• Specificity: CYP7B1 - cytochrome P450, family 7, subfamily B, polypeptide 1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: CYP7B1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 50 % glycerol
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CYP7B1 Antibody

• CBAS3
• CP7B
• Cytochrome P450 7B1
• cytochrome P450, family 7, subfamily B, polypeptide 1
• cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1,25-hydroxycholesterol 7-alpha-hydroxylase
• EC 1.14.13.100
• Oxysterol 7-alpha-hydroxylase
• oxysterol 7alpha-hydroxylase
• spastic paraplegia 5A (autosomal recessive)
• SPG5A

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CYP7B1 Antibody (H00009420-A01)(2)

Publications using H00009420-A01

• Dumas SN, Ntambi JM., et al. Increased hydrophilic plasma bile acids are correlated with protection from adiposity in skin-specific stearoyl-CoA desaturase-1 deficient mice. PLoS One 2018-07-02 [PMID: 29965978]

Bioinformatics

• Gene Symbol: CYP7B1
• Entrez:
  • 9420(Human)
• OMIM:
  • 231100(Human)
• Uniprot:
  • NP_004811(Human)