CH25H Antibody - BSA Free

Western Blot: CH25H Antibody [NBP3-05283] - Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: CH25H overexpressed HEK293T Lysate [10ug]. Lane 3: HeLa whole cell Lysate [10ug]. Primary Antibody: CH25H Antibody at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30min at RT. Block: Universal BlockOut Buffer. Predicted MW: ~31.7kDa. Observed MW: ~35kDa. Exposure: 3 seconds.

Immunohistochemistry: CH25H Antibody [NBP3-05283] - Tissue: Human Kidney. Fixative: None. Antigen Retrieval: HIER using Citrate Buffer for 20 minutes. Primary Antibody: CH25H Antibody at 1:100 for 30 min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP IgG Ready-to-Use for 8 minutes at RT. Counterstain: Hematoxylin. Substrate: DAB. Staining: (A) Negative control. (B) 20X. (C) 40X. Results: Shows strong cytoplasmic staining of renal tubular epithelium

Immunohistochemistry of CH25H Antibody. Tissue: Human Kidney. Fixative: None. Antigen Retrieval: HIER using Citrate Buffer for 20 minutes. Primary Antibody: Anti-CH25H at 1:100 for 30 min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP IgG Ready-to-Use for 8 minutes at RT. Counterstain: Hematoxylin. Substrate: DAB. Staining: (A) Negative control. (B) 20X. (C) 40X. Results: Shows strong cytoplasmic staining of renal tubular epithelium.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CH25H Antibody - BSA Free Summary

• Description: Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This affinity purified antibody is directed against human CH25H. This product was affinity purified from monospecific antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: CH25H Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: O95992)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CH25H
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 1:100
  • Western Blot 1:1000
• Application Notes: This product has been tested in ELISA, WB, and IHC. Expect a band at ~31.7kDa in western blot using appropriate tissues or lysates. Positive control used: Human Kidney Tissues in Immunohistochemistry.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence and 92% homology to pig, 91% homology to rat

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for CH25H Antibody - BSA Free

• C25H
• cholesterol 25-hydroxylase
• Cholesterol 25-monooxygenase
• EC 1.14.99.38
• h25OH

Background

CH25H that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CH25H
• Uniprot:
  • O95992(Human)