CXCR5 Antibody

Western Blot: CXCR5 Antibody [NBP2-16045] - Hep G2 10% SDS PAGE diluted at 1:1000

Immunohistochemistry-Paraffin: CXCR5 Antibody [NBP2-16045] - Mouse skin. CXCR5 stained by CXCR5 antibody [C1C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: CXCR5 Antibody [NBP2-16045] - CXCR5 antibody [C1C2], Internal detects CXCR5 protein at membrane on PC13 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC13 xenograft. CXCR5 antibody [C1C2], Internal dilution: 1:500.

Immunohistochemistry-Paraffin: CXCR5 Antibody [NBP2-16045] - Human kidney. CXCR5 stained by CXCR5 antibody [C1C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence: CXCR5 Antibody [NBP2-16045] - CXCR5 antibody [C1C2], Internal detects CXCR5 protein at cell membrane by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CXCR5 stained by CXCR5 antibody [C1C2], Internal (NBP2-16045) diluted at 1:500.Blue: Fluoroshield with DAPI .

Product Discontinued

CXCR5 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CXCR5. The exact sequence is proprietary.
• Localization: Cell membrane
• Predicted Species: Rat (92%), Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCR5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: ICC/IF-Assay dependent
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CXCR5 Antibody

• BLR1
• BLR1MDR-15
• Burkitt lymphoma receptor 1
• Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5)
• Burkitt lymphoma receptor 1, GTP-binding protein
• CD185 antigen
• CD185
• chemokine (C-X-C motif) receptor 5
• C-X-C chemokine receptor type 5
• CXCR5
• CXC-R5
• MDR15CXCR-5
• MGC117347
• Monocyte-derived receptor 15

Background

This gene was identified as a gene specifically expressed in Burkitt's lymphoma and lymphatic tissues. The protein encoded by this gene is predicted to be a seven transmembrane G protein- coupled receptor and belongs to the CXC chemokine receptor family. BLC, a B-lymphocyte chemoattractant, was identified to be a specific ligand for this receptor. Studies of this gene and its mouse conterpart strongly suggest the essential function of this gene in B cell migration and localization within specific anatomic compartments, such as follicles in lymph nodes as well as in spleen. Two alternatively spliced variants of this gene exist. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CXCR5
• Uniprot:
  • P32302()