CXCR4 Antibody (44708)

Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB171). The ND50 is typically 0.2-1.2 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: 44708
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR4 Antibody (44708) Summary

• Immunogen: 3T3 cells transfected with human CXCR4
• Specificity: Reacts specifically with human and non-human cells expressing human CXCR4 (fusin) as detected by flow cytometry. It will also react with cells expressing feline CXCR4 but not rat CXCR4. This antibody does not cross-react with other chemokine receptors.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR4
• Purity: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.2-1.2 ug/mL
• Control:

  CXCR4 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from ascites
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (44708)

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T cell tropic HIV‑1 and -2 viral entry into cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (MAB171)(12)

Publications using MAB171

• F Lüke, R Blazquez, RF Yamaci, X Lu, B Pregler, S Hannus, K Menhart, D Hellwig, HJ Wester, S Kropf, D Heudobler, J Grosse, J Moosbauer, M Hutterer, P Hau, MJ Riemenschn, M Bayerlová, A Bleckmann, B Polzer, T Bei beta barth, CA Klein, T Pukrop Isolated metastasis of an EGFR-L858R-mutated NSCLC of the meninges: the potential impact of CXCL12/CXCR4 axis in EGFRmut NSCLC in diagnosis, follow-up and treatment Oncotarget, 2018;9(27):18844-18857. 2018 [PMID: 29721166] (FCCS, Human)
• M Mushtaq, L Jensen, S Davidsson, OV Grygoruk, O Andrén, V Kashuba, E Kashuba The MRPS18-2 protein levels correlate with prostate tumor progression and it induces CXCR4-dependent migration of cancer cells Sci Rep, 2018;8(1):2268. 2018 [PMID: 29396484] (IHC-P, Human)
• Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27543332] (IHC-Fr, Human)
• DiCosmo-Ponticello C, Hoover D, Coffman F, Cohen S, Cohen M MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells. Cytokine, 2014;69(1):47-55. 2014 [PMID: 25022961] (Neutralization, Human)
• Calpe E, Purroy N, Carpio C, Abrisqueta P, Carabia J, Palacio C, Castellvi J, Crespo M, Bosch F ZAP-70 promotes the infiltration of malignant B-lymphocytes into the bone marrow by enhancing signaling and migration after CXCR4 stimulation. PLoS ONE, 2013;8(12):e81221. 2013 [PMID: 24312539] (Neutralization, Human)
• Weiss N, Deboux C, Chaverot N, Miller F, Baron-Van Evercooren A, Couraud P, Cazaubon S IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells. J Neuroimmunol, 0;223(1):131-4. 0 [PMID: 20400187] (Neutralization, Human)
• Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis. Eur. J. Immunol., 2008;38(11):3050-9. 2008 [PMID: 18924211] (Neutralization, Human)
• Ao M, Franco OE, Park D, Raman D, Williams K, Hayward SW Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium. Cancer Res., 2007;67(9):4244-53. 2007 [PMID: 17483336] (Western Blot, Human)
• Singh S, Singh UP, Grizzle WE, Lillard JW CXCL12-CXCR4 interactions modulate prostate cancer cell migration, metalloproteinase expression and invasion. Lab. Invest., 2004;84(12):1666-76. 2004 [PMID: 15467730] (Neutralization, Human)
• Wald O, Pappo O, Safadi R, Dagan-Berger M, Beider K, Wald H, Franitza S, Weiss I, Avniel S, Boaz P, Hanna J, Zamir G, Eid A, Mandelboim O, Spengler U, Galun E, Peled A Involvement of the CXCL12/CXCR4 pathway in the advanced liver disease that is associated with hepatitis C virus or hepatitis B virus. Eur. J. Immunol., 2004;34(4):1164-74. 2004 [PMID: 15048728] (IHC-P, Human)
• Hatse S, Princen K, Vermeire K, Gerlach LO, Rosenkilde MM, Schwartz TW, Bridger G, De Clercq E, Schols D Mutations at the CXCR4 interaction sites for AMD3100 influence anti-CXCR4 antibody binding and HIV-1 entry. FEBS Lett., 2003;546(2):300-6. 2003 [PMID: 12832058] (Flow Cytometry, Human)
• Nguyen DH, Taub D CXCR4 function requires membrane cholesterol: implications for HIV infection. J. Immunol., 2002;168(8):4121-6. 2002 [PMID: 11937572] (Flow Cytometry, Human)

FAQs for CXCR4 Antibody (MAB171). (Showing 1 - 1 of 1 FAQs).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4