CXCR4 Antibody (374606) [Unconjugated]

• Reactivity: Fe
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 374606
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR4 Antibody (374606) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with feline CXCR4
  Met1-Ser353
  Accession # P56498
• Specificity: Detects feline CXCR4. Stains feline CXCR4 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (374606) [Unconjugated]

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• pCXCR4
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4, also known as fusin and CD184, is a 7TM G protein-linked chemokine receptor. CXCR4 is expressed on B cells, monocytes, CD4⁺ T cells, colonic epithelial cells, and astrocytes and binds SDF-1/CXCL12. CXCR4 is up‑regulated on mammary tumors. CXCR4, in cooperation with CD134 and heparan sulfate proteoglycans, functions as a co-receptor for feline immunodeficiency virus (FIV) entry into cells. Feline CXCR4 shares 97%, 95%, 88%, and 90% aa sequence identity with canine, human, mouse, and rat CXCR4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (MAB4287)(1)

Publication using MAB4287

• JL Rojo, M Linari, KA Young, MC Peluffo Stromal-derived factor 1 directly promotes genes expressed within the ovulatory cascade in feline cumulus oocyte complexes J. Assist. Reprod. Genet., 2018-03-08;0(0):. 2018-03-08 [PMID: 29516335] (IHC, Feline)

FAQs for CXCR4 Antibody (MAB4287). (Showing 1 - 1 of 1 FAQ).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4
• Uniprot:
  • P56498()