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CXCR4 Antibody (247506R) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with mouse CXCR4 and eGFP was stained with (A) Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) or (B) Rat IgG2B isotype control antibody (Catalog # MAB0061) followed by APC-conjugated Anti-Rat IgG Secondary Antibody(Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with mouse CXCR4 and eGFP was stained with (A) Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) or (B) Rat IgG2B isotype control antibody (Catalog # ...read more
Mouse thymocytes were stained with Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R, filled histogram) or Rat IgG2B isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG ...read more
CXCR4 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
CXCR4 was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat ...read more
CXCR4 was detected in perfusion fixed frozen sections of mouse testis using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, ICC/IF
Clone
247506R
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB21651RU-100UG
Alexa Fluor 405
FAB21651RV-100UG
Alexa Fluor 488
FAB21651RG-100UG
Alexa Fluor 594
FAB21651RT-100UG
Alexa Fluor 647
FAB21651RR-100UG
Alexa Fluor 700
FAB21651RN-100UG
Alexa Fluor 750
FAB21651RS-100UG
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB21651R-SP
Carrier Free
FAB21651RT-100UG
Carrier Free
FAB21651RV-100UG
Carrier Free
FAB21651RS-100UG
Carrier Free
FAB21651RU-100UG
Carrier Free
FAB21651RR-100UG
Carrier Free
FAB21651RN-100UG
Carrier Free
FAB21651RG-100UG
100% Guarantee on every product

CXCR4 Antibody (247506R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Y3 rat myeloid cell line transfected with mouse CXCR4
Met1-Ser359
Accession # P70658
Specificity
Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 1-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (247506R) [Unconjugated]

  • CD184
  • chemokine (C-X-C motif) receptor 4
  • chemokine (C-X-C motif), receptor 4 (fusin)
  • C-X-C chemokine receptor type 4
  • CXCR4
  • CXC-R4
  • CXCR-4
  • D2S201E
  • D2S201ESDF-1 receptor
  • FB22
  • Fusin
  • HM89
  • HM89NPYRL
  • HSY3RR
  • LAP3
  • LAP-3
  • LCR1
  • LESTR
  • LESTRCD184 antigen
  • Leukocyte-derived seven transmembrane domain receptor
  • leukocyte-derived seven-transmembrane-domain receptor
  • lipopolysaccharide-associated protein 3
  • neuropeptide Y receptor Y3
  • NPY3R
  • NPY3RWHIM
  • NPYR
  • NPYRL
  • NPYY3R
  • seven transmembrane helix receptor
  • seven-transmembrane-segment receptor, spleen
  • Stromal cell-derived factor 1 receptor
  • WHIMS

Background

CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and-2 viral entry into cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCR1 Antibody (53504) [Unconjugated]
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CXCR4 Antibody (MAB21651R). (Showing 1 - 1 of 1 FAQ).

  1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
    • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB21651RU
Alexa Fluor 405 FAB21651RV
Alexa Fluor 488 FAB21651RG
Alexa Fluor 594 FAB21651RT
Alexa Fluor 647 FAB21651RR
Alexa Fluor 700 FAB21651RN
Alexa Fluor 750 FAB21651RS
Learn about Custom Antibody Labeling

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Blogs on CXCR4.

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Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.
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LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m...  Read full blog post.
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The CXCR4 (C-X-C chemokine receptor type 4) protein is one member of the G-protein coupled receptor (GPCR1) family. As a multipass membrane protein that is found in several tissues, it is the receptor for the C-X-C chemokine CXCL12/SDF-1. The CXCR4 li...  Read full blog post.
Understanding CXCR4 and SDF1
CXCR4 (C-X-C chemokine receptor type 4) is a member of the G-protein coupled receptor (GPCR1) family. It is expressed as a multipass membrane protein in several tissues where it acts as the receptor for the C-X-C chemokine CXCL12/SDF-1. This ligand in...  Read full blog post.
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