CXCR4 Antibody (247506) [Unconjugated]

CXCR4 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CXCR4 was detected in immersion fixed mouse neural progenitor cells using 10 µg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Mouse thymocytes were stained with (A) Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) or (B) Rat IgG2B isotype control antibody (Catalog # MAB0061) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B) and Rat anti-Mouse CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB116A). View our protocol for Staining Membrane-associated Proteins.

Recombinant Mouse CXCL12/SDF‑1 alpha (Catalog # 460-SD) chemo­attracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL12/ SDF‑1 alpha (10 ng/mL) is neutral­ized (green line) by increasing concentrations of Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651). The ND50 is typically 8-40 µg/mL.

CXCR4 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: Flow, IHC, B/N, CyTOF-ready, ICC/IF
• Clone: 247506
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR4 Antibody (247506) [Unconjugated] Summary

• Immunogen: Y3 rat myeloid cell line transfected with mouse CXCR4
  Met1-Ser359
  Accession # P70658
• Specificity: Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.
• Details of Functionality: ActivityAssaywCitation not found None None None None
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCR4
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 8-40 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (247506) [Unconjugated]

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and-2 viral entry into cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (MAB21651)(27)

Publications using MAB21651

• ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling J. Clin. Invest., 2019;130(0):2555-2570. 2019 [PMID: 31107242] (Neutralization, Mouse)
• MJ Lopez, Y Seyed-Raza, A Jamali, DL Harris, P Hamrah The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea Invest. Ophthalmol. Vis. Sci., 2018;59(13):5671-5681. 2018 [PMID: 30489627] (Flow Cytometry, Mouse)
• H Nagashima, M Shinoda, K Honda, N Kamio, A Hasuike, N Sugano, Y Arai, S Sato, K Iwata CXCR4 signaling contributes to alveolar bone resorption in Porphyromonas gingivalis-induced periodontitis in mice J Oral Sci, 2017;0(0):. 2017 [PMID: 29093284] (Neutralization, Mouse)
• M Zhang, L Qiu, Y Zhang, D Xu, JC Zheng, L Jiang CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells Sci Rep, 2017;7(1):8289. 2017 [PMID: 28811579] (Bioassay, Human)
• A Saha, S Ahn, J Blando, F Su, MG Kolonin, J DiGiovanni Proinflammatory CXCL12-CXCR4/CXCR7 signaling axis drives Myc-induced prostate cancer in obese mice Cancer Res., 2017;0(0):. 2017 [PMID: 28687617] (IHC, Mouse)
• H Nagashima, M Shinoda, K Honda, N Kamio, M Watanabe, T Suzuki, N Sugano, S Sato, K Iwata CXCR4 signaling in macrophages contributes to periodontal mechanical hypersensitivity in Porphyromonas gingivalis-induced periodontitis in mice Mol Pain, 2017;13(0):1744806916689. 2017 [PMID: 28326928] (Neutralization, Mouse)
• N Smith, N Pietrancos, S Davidson, J Dutrieux, L Chauveau, P Cutolo, M Dy, D Scott-Alga, B Manoury, O Zirafi, I McCort-Tra, T Durroux, F Bachelerie, O Schwartz, J Münch, A Wack, S Nisole, JP Herbeuval Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement Nat Commun, 2017;8(0):14253. 2017 [PMID: 28181493] (ICC, Mouse)
• KI Cho, D Yoon, S Qiu, Z Danziger, WM Grill, WC Wetsel, PA Ferreira Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes Dis Model Mech, 2017;0(0):. 2017 [PMID: 28100513] (ICC, Mouse)
• A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways Sci Rep, 2016;6(0):25104. 2016 [PMID: 27157261] (IHC, Mouse)
• Chen Q, Zhang M, Li Y, Xu D, Wang Y, Song A, Zhu B, Huang Y, Zheng J CXCR7 Mediates Neural Progenitor Cells Migration to CXCL12 Independent of CXCR4. Stem Cells, 2015;33(8):2574-85. 2015 [PMID: 25833331] (IHC, Mouse)
• Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery. J Leukoc Biol, 2015;97(3):583-98. 2015 [PMID: 25583577] (Flow Cytometry, Mouse)
• Butschkau, Antje, Wagner, Nana-Mar, Genz, Berit, Vollmar, Brigitte Protein z exerts pro-angiogenic effects and upregulates CXCR4. PLoS ONE, 2014;9(12):e113554. 2014 [PMID: 25474349] (ICC, Human)
• DeNiro M, Al-Mohanna F Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment. PLoS ONE, 2014;9(7):e101602. 2014 [PMID: 25050547] (IHC-P, Mouse)
• Zgraggen, Silvana, Huggenberger, Reto, Kerl, Katrin, Detmar, Michael An important role of the SDF-1/CXCR4 axis in chronic skin inflammation. PLoS ONE, 2014;9(4):e93665. 2014 [PMID: 24695674] (IHC, Mouse)
• Roy, Lopamudr, Sahraei, Mahnaz, Schettini, Jorge L, Gruber, Helen E, Besmer, Dahlia M, Mukherjee, Pinku Systemic neutralization of IL-17A significantly reduces breast cancer associated metastasis in arthritic mice by reducing CXCL12/SDF-1 expression in the metastatic niches. BMC Cancer, 2014;14(0):225. 2014 [PMID: 24674692] (Neutralization, Mouse)
• Kavanagh D, Yemm A, Zhao Y, Frampton J, Kalia N Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo. PLoS ONE, 2013;8(3):e59150. 2013 [PMID: 23554986] (Flow Cytometry, Mouse)
• Yang, Qi-En, Kim, Dongwon, Kaucher, Amy, Oatley, Melissa, Oatley, Jon M CXCL12-CXCR4 signaling is required for the maintenance of mouse spermatogonial stem cells. J Cell Sci, 2013;126(0):1009-20. 2013 [PMID: 23239029] (Flow Cytometry, Mouse)
• Kohler T, Reizis B, Johnson RS, Weighardt H, Forster I Influence of hypoxia-inducible factor 1-alpha on dendritic cell differentiation and migration. Eur. J. Immunol., 2012;42(5):1226-36. 2012 [PMID: 22539295] (Flow Cytometry, Mouse)
• Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury. PLoS ONE, 2012;7(4):e34608. 2012 [PMID: 22511954] (Neutralization, Mouse)
• Salvucci O, Jiang K, Gasperini P MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1. Haematologica, 2012;97(6):818-26. 2012 [PMID: 22271895] (Flow Cytometry, Mouse)
• Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation. J. Immunol., 2011;187(9):4835-43. 2011 [PMID: 21930960] (Flow Cytometry, Mouse)
• Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs. Biochem. Biophys. Res. Commun., 2010;401(4):509-15. 2010 [PMID: 20869949] (Neutralization, Mouse)
• Strasser GA, Kaminker JS, Tessier-Lavigne M Microarray analysis of retinal endothelial tip cells identifies CXCR4 as a mediator of tip cell morphology and branching. Blood, 2010;115(24):5102-10. 2010 [PMID: 20154215] (Neutralization, Mouse)
• Sengupta N, Afzal A, Caballero S, Chang KH, Shaw LC, Pang JJ, Bond VC, Bhutto I, Baba T, Lutty GA, Grant MB Paracrine modulation of CXCR4 by IGF-1 and VEGF: implications for choroidal neovascularization. Invest. Ophthalmol. Vis. Sci., 2010;51(5):2697-704. 2010 [PMID: 20007826] (Neutralization, Mouse)
• Schroeter MR, Humboldt T, Schafer K, Konstantinides S Rosuvastatin reduces atherosclerotic lesions and promotes progenitor cell mobilisation and recruitment in apolipoprotein E knockout mice. Atherosclerosis, 2009;205(1):63-73. 2009 [PMID: 19111832] (IHC, Mouse)
• Feng W, McCabe NP, Mahabeleshwar GH, Somanath PR, Phillips DR, Byzova TV The angiogenic response is dictated by beta3 integrin on bone marrow-derived cells. J. Cell Biol., 2008;183(6):1145-57. 2008 [PMID: 19075116] (IHC-Fr, Mouse)
• Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells. Cancer Res., 2008;68(6):1889-95. 2008 [PMID: 18339870] (IHC-Fr, Mouse)

Review for CXCR4 Antibody (MAB21651) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous IF Mouse 02/19/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22752632
• Species: Mouse

Blocking

• Blocking Details: See PMID 22752632

Details

• Detection Notes: See PMID 22752632

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID: 22752632

FAQs for CXCR4 Antibody (MAB21651). (Showing 1 - 1 of 1 FAQs).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4
• Uniprot:
  • P70658()