CXCR3 Antibody (49801) [Fluorescein]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB160F) and Mouse Anti-Human CD3e Alexa Fluor® 700-conjugated Monoclonal Antibody (FAB100N). Quadrant markers were set based on control antibody staining (IC002F). View our protocol for Staining Membrane-associated Proteins.

RPMI8226 human myeloma cell line was stained with Mouse Anti-Human CXCR3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB160F, filled histogram) or Mouse IgG1 isotype control antibody (IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 49801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

CXCR3 Antibody (49801) [Fluorescein] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR3
  Met1-Leu368
  Accession # P49682
• Specificity: Detects human CXCR3.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR3 Antibody (49801) [Fluorescein]

• CD183 antigen
• CD183
• chemokine (C-X-C motif) receptor 3
• chemokine (C-X-C) receptor 3
• CKR-L2IP-10 receptor
• CMKAR3
• C-X-C chemokine receptor type 3
• CXCR3
• CXC-R3
• CXCR-3
• G protein-coupled receptor 9CD182
• GPR9
• GPR9Mig-R
• Interferon-inducible protein 10 receptor
• IP10 receptor
• IP10-R
• Mig receptor
• MigR

Background

CXCR3 is a G protein-coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10), and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR3 Antibody (FAB160F)(24)

Publications using FAB160F

• L Amniai, C Ple, M Barrier, P de Nadai, P Marquillie, H Vorng, C Chenivesse, A Tsicopoulo, C Duez Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine International Journal of Molecular Sciences, 2021-04-09;22(8):. 2021-04-09 [PMID: 33918621] (Flow Cytometry, Human)
• K Kurihara, T Fujiyama, P Phadungsak, T Ito, Y Tokura Significance of IL-17A-producing CD8+CD103+ skin resident memory T cells in psoriasis lesion and their possible relationship to clinical course J. Dermatol. Sci., 2019-06-20;95(1):21-27. 2019-06-20 [PMID: 31300254] (Flow Cytometry, Human)
• Zaidoon Al-Jaderi Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity Front Immunol, 2016-10-19;7(0):437. 2016-10-19 [PMID: 27807435] (Flow Cytometry, Human)
• A distinct plasmablast and na�ve B-cell phenotype in primary immune thrombocytopenia Haematologica, 2016-03-11;101(6):698-706. 2016-03-11 [PMID: 26969086] (Flow Cytometry, Human)
• Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1. J Immunol, 2014-05-16;192(12):5776-88. 2014-05-16 [PMID: 24835401] (Flow Cytometry, Human)
• Aruga , Atsushi, Takeshita , Nobuhiro, Kotera , Yoshihit, Okuyama , Ryuji, Matsushita , Norimasa, Ohta , Takehiro, Takeda , Kazuyosh, Yamamoto , Masakazu Phase I clinical trial of multiple-peptide vaccination for patients with advanced biliary tract cancer. J Transl Med, 2014-03-07;12(0):61. 2014-03-07 [PMID: 24606884] (Flow Cytometry, Human)
• Chihara , Norio, Aranami , Toshimas, Oki , Shinji, Matsuoka , Takako, Nakamura , Masakazu, Kishida , Hitaru, Yokoyama , Kazumasa, Kuroiwa , Yoshiyuk, Hattori , Nobutaka, Okamoto , Tomoko, Murata , Miho, Toda , Tatsushi, Miyake , Sachiko, Yamamura , Takashi Plasmablasts as migratory IgG-producing cells in the pathogenesis of neuromyelitis optica. PLoS ONE, 2013-12-10;8(12):e83036. 2013-12-10 [PMID: 24340077] (Flow Cytometry, Human)
• Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation. J Immunol, 2013-02-27;190(7):3696-705. 2013-02-27 [PMID: 23447686] (Flow Cytometry, Human)
• Wang W, Yu D, Sarnaik A, Yu B, Hall M, Morelli D, Zhang Y, Zhao X, Weber J Biomarkers on melanoma patient T cells associated with ipilimumab treatment. J Transl Med, 2012-07-12;10(0):146. 2012-07-12 [PMID: 22788688] (Flow Cytometry, Human)
• Morandi F, Prigione I, Airoldi I Human TCR gamma delta+ T cells represent a novel target for IL-27 activity. Eur. J. Immunol., 2012-06-01;42(6):1547-52. 2012-06-01 [PMID: 22678908] (Flow Cytometry, Human)
• Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA Identification of human NK17/NK1 cells. PLoS ONE, 2011-10-21;6(10):e26780. 2011-10-21 [PMID: 22039549] (Flow Cytometry, Human)
• Zhang HH, Song K, Rabin RL, Hill BJ, Perfetto SP, Roederer M, Douek DC, Siegel RM, Farber JM CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response. J. Immunol., 2010-10-27;185(11):6646-63. 2010-10-27 [PMID: 20980630] (Flow Cytometry, Human)
• Datta D, Banerjee P, Gasser M CXCR3-B can mediate growth-inhibitory signals in human renal cancer cells by down-regulating the expression of heme oxygenase-1. J. Biol. Chem., 2010-09-20;285(47):36842-8. 2010-09-20 [PMID: 20855888] (Flow Cytometry, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Flow Cytometry, Human)
• Pedersen AW, Holmstrom K, Jensen SS, Fuchs D, Rasmussen S, Kvistborg P, Claesson MH, Zocca MB Phenotypic and functional markers for 1alpha,25-dihydroxyvitamin D(3)-modified regulatory dendritic cells. Clin. Exp. Immunol., 2009-07-01;157(1):48-59. 2009-07-01 [PMID: 19659770] (Flow Cytometry, Human)
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-12-01;181(11):8053-67. 2008-12-01 [PMID: 19017998] (Flow Cytometry, Human)
• Meiser A, Mueller A, Wise EL, McDonagh EM, Petit SJ, Saran N, Clark PC, Williams TJ, Pease JE The chemokine receptor CXCR3 is degraded following internalization and is replenished at the cell surface by de novo synthesis of receptor. J. Immunol., 2008-05-15;180(10):6713-24. 2008-05-15 [PMID: 18453591] (ICC, Human)
• Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis. J. Leukoc. Biol., 2007-07-05;82(4):946-55. 2007-07-05 [PMID: 17615381] (Flow Cytometry, Human)
• Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis. Allergy, 2007-02-01;62(2):170-7. 2007-02-01 [PMID: 17298426] (Flow Cytometry, Human)
• Mamoni RL, Blotta MH Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis. Cytokine, 2006-10-11;35(3):207-16. 2006-10-11 [PMID: 17045486] (Flow Cytometry, Human)
• Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Flow Cytometry, Human)
• Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 2004-06-01;112(2):274-89. 2004-06-01 [PMID: 15147571] (Flow Cytometry, Human)
• D'Ambrosio D Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes. Eur. J. Immunol., 2002-10-01;32(10):2934-43. 2002-10-01 [PMID: 12355447] (Flow Cytometry, Human)
• Janatpour MJ, Hudak S, Sathe M, McEvoy LM Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment. J. Exp. Med., 2001-11-05;194(9):1375-84. 2001-11-05 [PMID: 11696601] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CXCR3
• Uniprot:
  • P49682()