Human CXCR2/IL-8RB ELISA Kit (Colorimetric) Summary
| Specificity |
This kit recognizes Human CXCR2/IL-8RB in samples. No significant cross-reactivity or interference between Human CXCR2/IL-8RB and analogues was observed. |
| Standard Curve Range |
0.78 - 50 ng/mL |
| Sensitivity |
0.47 ng/mL |
| Assay Type |
Sandwich-ELISA |
| Inter-Assay |
4.38 |
| Intra-Assay |
4.57 |
| Spike Recovery |
89 - 109 % |
| Sample Volume |
100 ul |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CXCR2 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Human CXCR2/IL-8RB ELISA Kit (Colorimetric)
Background
Chemokine (Chemoattractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells. Chemokine receptors (CXCR) belong to the super-family of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and proliferation and migration of immature neurons, glia and their precursors. Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases several subtypes of CXCR have been characterized. Activation of na
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for CXCR2/IL-8RB ELISA Kit (NBP3-06837). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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