CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free

Immunohistochemistry-Paraffin: CXCR2/IL-8 RB Antibody (5E8-C7-F10) [NBP1-43338] - CXCR2positive breast cancer cells in the human brain. CXCR2-red fluorochrome, nucleus-DAPI. Antibody used at dilution of 1:100. This image was submitted via customer Review.

Flow Cytometry: CXCR2/IL-8 RB Antibody (5E8-C7-F10) [NBP1-43338] - Analysis using the PE conjugate of NBP1-43338. Staining of normal human peripheral blood cells with Anti-Human CD4 APC (left) or Anti-Human CD56 (NCAM) APC (right) and Anti-Human CD182 (CXCR2) PE. Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: CXCR2/IL-8 RB Antibody (5E8-C7-F10) [NBP1-43338] - Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (left) or 0.25 ug of Anti-Human CD182 (CXCR2) Purified (right) followed by F(ab')2 Anti-Mouse IgG PE . Cells in the large scatter population were used for analysis.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: 5E8-C7-F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free Summary

• Immunogen: The immunogen for this antibody was CD182.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CXCR2/IL-8RB Antibody (5E8-C7-F10) - BSA Free

• CD182 antigen
• CD182
• CDw128b
• chemokine (C-X-C motif) receptor 2
• chemokine (CXC) receptor 2
• CMKAR2
• C-X-C chemokine receptor type 2
• CXCR2 gene for IL8 receptor type B
• CXCR2
• CXC-R2
• CXCR-2
• GRO/MGSA receptor
• High affinity interleukin-8 receptor B
• IL-8 RB
• IL-8 receptor type 2
• IL-8R B
• IL8R2
• IL8RA
• IL8RB
• interleukin 8 receptor B
• interleukin 8 receptor type 2
• interleukin-8 receptor type B

Background

The 5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8Rbeta). CD182 is a 67-70 kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8Ralpha) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of its ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, -beta and -gamma, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CXCR2/IL-8RB Antibody (NBP1-43338) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ilya Ulasov IHC-P Human 02/22/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: breast cancer in the human brain ( metastases)
• Species: Human

Comments

• Comments: standard protocol, dilution 1:100

Bioinformatics

• Gene Symbol: CXCR2