CXCR2/IL-8RB Antibody (547) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CXCR2/IL-8RB Antibody (547) [NBP2-43810] - CXCR2 antibody [547] detects CXCR2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCR2 stained by CXCR2 antibody [547] diluted at 1:2000.Blue: Hoechst 33342 staining. Scale bar= 10um.

Non-transfected (-) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [GT547] (NBP2-43810) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF
• Clone: 547
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CXCR2/IL-8RB Antibody (547) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CXCR2/IL-8RB Antibody (547) - Azide and BSA Free (NBP2-43810) is a monoclonal antibody validated for use in WB and ICC/IF. Anti-CXCR2/IL-8RB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human CXCR2/IL-8RB. The exact sequence is proprietary.
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CXCR2/IL-8RB Antibody (547) - Azide and BSA Free

• CD182 antigen
• CD182
• CDw128b
• chemokine (C-X-C motif) receptor 2
• chemokine (CXC) receptor 2
• CMKAR2
• C-X-C chemokine receptor type 2
• CXCR2 gene for IL8 receptor type B
• CXCR2
• CXC-R2
• CXCR-2
• GRO/MGSA receptor
• High affinity interleukin-8 receptor B
• IL-8 RB
• IL-8 receptor type 2
• IL-8R B
• IL8R2
• IL8RA
• IL8RB
• interleukin 8 receptor B
• interleukin 8 receptor type 2
• interleukin-8 receptor type B

Background

Chemokine (Chemoattractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells. Chemokine receptors (CXCR) belong to the super-family of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and proliferation and migration of immature neurons, glia and their precursors. Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases several subtypes of CXCR have been characterized. Activation of na

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR2/IL-8RB Antibody (NBP2-43810)(2)

Publications using NBP2-43810

• Jayatilaka H, Tyle P, Chen JJ et al. Synergistic IL-6 and IL-8 paracrine signalling pathway infers a strategy to inhibit tumour cell migration. Nat Commun. 2017 May 26 [PMID: 28548090] (WB, Mouse)
• H Jayatilaka, P Tyle, JJ Chen, M Kwak, J Ju, HJ Kim, JSH Lee, PH Wu, DM Gilkes, R Fan, D Wirtz Synergistic IL-6 and IL-8 paracrine signalling pathway infers a strategy to inhibit tumour cell migration Nat Commun, 2017-05-26;8(0):15584. 2017-05-26 [PMID: 28548090] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CXCR2