CXCR2/IL-8RB Antibody (242216) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouseCXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).

CXCR2/IL‑8 RB was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell sufaces in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Recombinant Mouse CXCL2/MIP‑2 (Catalog #452-M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164). The ND50 is typically 15-50 µg/mL.

• Reactivity: Mu
• Applications: Flow, IHC, B/N, CyTOF-ready
• Clone: 242216
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR2/IL-8RB Antibody (242216) [Unconjugated] Summary

• Immunogen: C6 rat glioma cell line transfected with mouse CXCR2
  Met1-Leu359
  Accession # P35343
• Specificity: Stains mouse CXCR2 transfected cells but not CXCR1 transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • Neutralization 15-50 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR2/IL-8RB Antibody (242216) [Unconjugated]

• CD182 antigen
• CD182
• CDw128b
• chemokine (C-X-C motif) receptor 2
• chemokine (CXC) receptor 2
• CMKAR2
• C-X-C chemokine receptor type 2
• CXCR2 gene for IL8 receptor type B
• CXCR2
• CXC-R2
• CXCR-2
• GRO/MGSA receptor
• High affinity interleukin-8 receptor B
• IL-8 RB
• IL-8 receptor type 2
• IL-8R B
• IL8R2
• IL8RA
• IL8RB
• interleukin 8 receptor B
• interleukin 8 receptor type 2
• interleukin-8 receptor type B

Background

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha , GRO beta , GRO gamma , NAP-2, and ENA-78.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR2/IL-8RB Antibody (MAB2164)(49)

Publications using MAB2164

• Zhao, YF;Zuo, ZA;Li, ZY;Yuan, Y;Hong, SC;Fu, WG;Zhou, B;Wang, LX; Integrated multi-omics profiling reveals neutrophil extracellular traps potentiate Aortic dissection progression Nature communications 2024-12-30 [PMID: 39737994] (In vivo assay, Mouse)
• Napoli, M;Immler, R;Rohwedder, I;Lupperger, V;Pfabe, J;Gonzalez Pisfil, M;Yevtushenko, A;Vogl, T;Roth, J;Salvermoser, M;Dietzel, S;Slak Rupnik, M;Marr, C;Walzog, B;Sperandio, M;Pruenster, M; Cytosolic S100A8/A9 promotes Ca2+ supply at LFA-1 adhesion clusters during neutrophil recruitment eLife 2024-12-19 [PMID: 39699020] (Bioassay, Flow Cytometry, Mouse)
• Xue, P;Lv, L;Liu, L;Xu, Y;Zhou, C;Wang, Y; Unveiling the role of CXCL8/CXCR2 in intervertebral disc degeneration: A path to promising therapeutic strategies Journal of orthopaedic translation 2024-11-01 [PMID: 39444419] (Immunohistochemistry, Western Blot, Immunocytochemistry, Human, Rat)
• Wu, F;Han, Y;Xiong, Q;Tang, H;Shi, J;Yang, Q;Li, X;Jia, H;Qian, J;Dong, Y;Li, T;Gao, Y;Qian, Z;Wang, H;Wang, T; Cerebral Endothelial CXCR2 Promotes Neutrophil Transmigration into Central Nervous System in LPS-Induced Septic Encephalopathy Biomedicines 2024-07-11 [PMID: 39062109] (Flow Cytometry, Mouse, Transgenic Mouse)
• Sawant, KV;Sepuru, KM;Penaranda, B;Lowry, E;Garofalo, RP;Rajarathnam, K; Chemokine Cxcl1-Cxcl2 heterodimer is a potent neutrophil chemoattractant Journal of leukocyte biology 2023-08-25 [PMID: 37625009] (Flow Cytometry, Mouse)
• S Luo, C Liao, L Zhang, C Ling, X Zhang, P Xie, G Su, Z Chen, L Zhang, T Lai, J Tang METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling Cell Reports, 2023-03-14;42(3):112259. 2023-03-14 [PMID: 36920907] (Neutralization, Mouse)
• P Pavlidis, A Tsakmaki, E Pantazi, K Li, D Cozzetto, J Digby-Bell, F Yang, JW Lo, E Alberts, ACC Sa, U Niazi, J Friedman, AK Long, Y Ding, CD Carey, C Lamb, M Saqi, M Madgwick, L Gul, A Treveil, T Korcsmaros, TT Macdonald, GM Lord, G Bewick, N Powell Interleukin-22 regulates neutrophil recruitment in ulcerative colitis and is associated with resistance to ustekinumab therapy Nature Communications, 2022-10-03;13(1):5820. 2022-10-03 [PMID: 36192482] (In Vivo, Mouse)
• A Shimada, S Hasegawa-I Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice Toxicology reports, 2021-03-10;8(0):520-528. 2021-03-10 [PMID: 33747797] (IHC, Mouse)
• Marie Vandestienne, Yujiao Zhang, Icia Santos-Zas, Rida Al-Rifai, Jeremie Joffre, Andreas Giraud et al. TREM-1 orchestrates angiotensin II–induced monocyte trafficking and promotes experimental abdominal aortic aneurysm Journal of Clinical Investigation 2021-01-19 [PMID: 33258804] (Flow Cytometry, Mouse)
• Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al. Cytotoxic T cells swarm by homotypic chemokine signalling eLife 10/13/2020 [PMID: 33046212]
• K Ogawa, K Asano, S Yotsumoto, T Yamane, M Arita, Y Hayashi, H Harada, C Makino-Oka, H Fukuyama, K Kondo, T Yamasoba, M Tanaka Frontline Science:�Conversion of neutrophils into atypical Ly6G+ SiglecF+ immune cells with neurosupportive potential in olfactory neuroepithelium J. Leukoc. Biol., 2020-07-29;0(0):. 2020-07-29 [PMID: 32725843] (Flow Cytometry, Mouse)
• RS Aquino, A Hayashida, PW Park Host syndecan-1 promotes listeriosis by inhibiting intravascular neutrophil extracellular traps PLoS Pathog., 2020-05-26;16(5):e1008497. 2020-05-26 [PMID: 32453780] (In Vivo, Mouse)
• Z Zhang, L Dong, A Jia, X Chen, Q Yang, Y Wang, Y Wang, R Liu, Y Cao, Y He, Y Bi, G Liu Glucocorticoids Promote the Onset of Acute Experimental Colitis and Cancer by Upregulating mTOR Signaling in Intestinal Epithelial Cells Cancers (Basel), 2020-04-11;12(4):. 2020-04-11 [PMID: 32290362] (Flow Cytometry, Mouse)
• MY Park, HS Kim, HY Lee, BA Zabel, YS Bae Novel CD11b+Gr-1+Sca-1+ myeloid cells drive mortality in bacterial infection Sci Adv, 2020-01-22;6(4):eaax8820. 2020-01-22 [PMID: 32010784] (Flow Cytometry, Mouse)
• M Mohammad, MT Nguyen, C Engdahl, M Na, A Jarneborn, Z Hu, A Karlsson, R Pullerits, A Ali, F Götz, T Jin The YIN and YANG of lipoproteins in developing and preventing infectious arthritis by Staphylococcus aureus PLoS Pathog., 2019-06-21;15(6):e1007877. 2019-06-21 [PMID: 31226163] (Neutralization, Mouse)
• ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling J. Clin. Invest., 2019-05-20;130(0):2555-2570. 2019-05-20 [PMID: 31107242] (Neutralization, Mouse)
• K Kozasa, S Mabuchi, Y Matsumoto, H Kuroda, E Yokoi, N Komura, M Kawano, R Takahashi, T Sasano, K Shimura, M Kodama, K Hashimoto, K Sawada, K Nagasaka, T Kimura Estrogen stimulates female cancer progression by inducing myeloid-derived suppressive cells: investigations on pregnant and non-pregnant experimental models Oncotarget, 2019-03-08;10(20):1887-1902. 2019-03-08 [PMID: 30956772] (Neutralization, Mouse)
• Wenting Liao, Michael J. Overman, Adam T. Boutin, Xiaoying Shang, Di Zhao, Prasenjit Dey et al. KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer Cancer Cell 2019-04-01 [PMID: 30905761]
• Tamara Girbl, Tchern Lenn, Lorena Perez, Loïc Rolas, Anna Barkaway, Aude Thiriot et al. Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis Immunity 2018-12-18 [PMID: 30446388]
• Mollaoglu G, Jones A, Wait SJ et al. The Lineage-Defining Transcription Factors SOX2 and NkX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment. Immunity. 10/16/2018 [PMID: 30332632]
• Pratik P. Vikhe, Tom Purnell, Steve D.M. Brown, Derek W. Hood Cellular content plays a crucial role in Non-typeable Haemophilus influenzae infection of preinflamed Junbo mouse middle ear Cellular Microbiology 2019-01-01 [PMID: 30265765]
• T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment Oncotarget, 2018-01-29;9(13):11209-11226. 2018-01-29 [PMID: 29541408] (Flow Cytometry, Mouse)
• K Watanabe, CA Gilchrist, MJ Uddin, SL Burgess, MM Abhyankar, SN Moonah, Z Noor, JR Donowitz, BN Schneider, T Arju, E Ahmed, M Kabir, M Alam, R Haque, P Pramoonjag, B Mehrad, WA Petri Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis PLoS Pathog., 2017-08-17;13(8):e1006513. 2017-08-17 [PMID: 28817707] (Neutralization, Mouse)
• C Zhang, S Wang, J Li, W Zhang, L Zheng, C Yang, T Zhu, R Rong The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury Cell Death Dis, 2017-03-23;8(3):e2695. 2017-03-23 [PMID: 28333137] (Flow Cytometry, Mouse)
• Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy. J Biol Chem, 2014-12-11;290(4):2053-68. 2014-12-11 [PMID: 25505239] (IHC, Mouse)
• Wang X, Bi Y, Xue L, Liao J, Chen X, Lu Y, Zhang Z, Wang J, Liu H, Yang H, Liu G The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation. Mol Cell Biol, 2014-12-01;35(3):598-609. 2014-12-01 [PMID: 25452304] (Neutralization, Mouse)
• Labelle , Myriam, Begum , Shahinoo, Hynes , Richard Platelets guide the formation of early metastatic niches. Proc Natl Acad Sci U S A, 2014-07-14;111(30):E3053-61. 2014-07-14 [PMID: 25024172] (Neutralization, Mouse)
• Steven L. Highfill, Yongzhi Cui, Amber J. Giles, Jillian P. Smith, Hua Zhang, Elizabeth Morse et al. Disruption of CXCR2-Mediated MDSC Tumor Trafficking Enhances Anti-PD1 Efficacy Science Translational Medicine 2014-05-21 [PMID: 24848257]
• Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner R, Moschen A, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf A, Wolf D, Eller P, Ritsch A, Weiss G Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2. EMBO Mol Med, 2014-04-06;6(6):810-20. 2014-04-06 [PMID: 24755316] (Flow Cytometry, Mouse)
• Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments. Exp Cell Res, 2014-02-22;323(1):7-27. 2014-02-22 [PMID: 24569142] (Functional Assay, Mouse)
• Zhang Y, Bi Y, Yang H, Chen X, Liu H, Lu Y, Zhang Z, Liao J, Yang S, Chu Y, Yang R, Liu G mTOR limits the recruitment of CD11b+Gr1+Ly6Chigh myeloid-derived suppressor cells in protecting against murine immunological hepatic injury. J Leukoc Biol, 2014-02-25;95(6):961-70. 2014-02-25 [PMID: 24569105] (Neutralization, Mouse)
• Sun B, Hu X, Liu G, Ma B, Xu Y, Yang T, Shi J, Yang F, Li H, Zhang L, Zhao Y Phosphatase Wip1 negatively regulates neutrophil migration and inflammation. J Immunol, 2014-01-06;192(3):1184-95. 2014-01-06 [PMID: 24395919] (Flow Cytometry, Mouse)
• Bi Y, Zhou J, Yang H, Wang X, Zhang X, Wang Q, Wu X, Han Y, Song Y, Tan Y, Du Z, Yang H, Zhou D, Cui Y, Zhou L, Yan Y, Zhang P, Guo Z, Wang X, Liu G, Yang R IL-17A produced by neutrophils protects against pneumonic plague through orchestrating IFN-gamma-activated macrophage programming. J Immunol, 2013-12-13;192(2):704-13. 2013-12-13 [PMID: 24337746] (Flow Cytometry, Mouse)
• Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta. Int J Cancer, 2013-10-31;134(6):1346-58. 2013-10-31 [PMID: 24154944] (Neutralization, Mouse)
• Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J Immunol, 2013-04-15;190(10):5329-36. 2013-04-15 [PMID: 23589623] (Neutralization, Mouse)
• Zhang Q, Coveney A, Yu S, Liu J, Li Y, Blankson S, Redmond H, Wang J, Wang J Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection. Eur J Immunol, 2013-03-22;43(5):1322-32. 2013-03-22 [PMID: 23404483] (Flow Cytometry, Mouse)
• Roy M, Richard JF, Dumas A, Vallieres L CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis. J Neuroinflammation, 2012-01-23;9(0):18. 2012-01-23 [PMID: 22269426] (Neutralization, Mouse)
• Halpern Jennifer L, Kilbarger Amy, Lynch Conor C. Mesenchymal stem cells promote mammary cancer cell migRation in vitro via the CXCR2 receptor. Cancer Letters 1/1/2011 [PMID: 21601983]
• Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines. J. Lipid Res., 2011-04-26;52(7):1307-18. 2011-04-26 [PMID: 21521824] (Neutralization, Mouse)
• von Vietinghoff S, Asagiri M, Azar D Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice. J. Immunol., 2010-06-02;185(1):670-8. 2010-06-02 [PMID: 20519647] (Neutralization, Mouse)
• Traylor ZP, Yu EN, Davis IC Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice. Am. J. Physiol. Lung Cell Mol. Physiol., 2009-12-04;298(3):L437-45. 2009-12-04 [PMID: 19965982] (Neutralization, Mouse)
• Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9. 2009-03-16 [PMID: 19289819] (Neutralization, Mouse)
• Omari KM, Lutz SE, Santambrogio L, Lira SA, Raine CS Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1. Am. J. Pathol., 2008-12-18;174(1):164-76. 2008-12-18 [PMID: 19095949] (IHC-Fr, Mouse)
• Sawanobori Y, Ueha S, Kurachi M, Shimaoka T, Talmadge JE, Abe J, Shono Y, Kitabatake M, Kakimi K, Mukaida N, Matsushima K Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice. Blood, 2008-03-28;111(12):5457-66. 2008-03-28 [PMID: 18375791] (Flow Cytometry, Mouse)
• Nomellini V, Faunce DE, Gomez CR, Kovacs EJ An age-associated increase in pulmonary inflammation after burn injury is abrogated by CXCR2 inhibition. J. Leukoc. Biol., 2008-03-13;83(6):1493-501. 2008-03-13 [PMID: 18319289] (Neutralization, Mouse)
• Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling. Blood, 2008-01-16;111(7):3343-54. 2008-01-16 [PMID: 18199827] (Neutralization, Mouse)
• Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice. Eur. J. Immunol., 2007-10-01;37(10):2753-63. 2007-10-01 [PMID: 17823985] (Neutralization, Mouse)
• Mohsenin A, Mi T, Xia Y, Kellems RE, Chen JF, Blackburn MR Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-06-29;293(3):L753-61. 2007-06-29 [PMID: 17601796] (IHC, Mouse)
• Yu JJ, Ruddy MJ, Wong GC, Sfintescu C, Baker PJ, Smith JB, Evans RT, Gaffen SL An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals. Blood, 2007-01-03;109(9):3794-802. 2007-01-03 [PMID: 17202320] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CXCR2
• Uniprot:
  • P35343()