CXCR2/IL-8RB Antibody (242216) [Phycoerythrin]

Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rat Anti-Mouse CXCR2/IL-8 RB PE-conjugated Monoclonal Antibody (Catalog # FAB2164P) or (B) Rat IgG1Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB PE-conjugated Monoclonal Antibody (Catalog # FAB2164P). Quadrant markers were set based on control antibody staining (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 242216
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

CXCR2/IL-8RB Antibody (242216) [Phycoerythrin] Summary

• Immunogen: C6 rat glioma cell line transfected with mouse CXCR2
  Met1-Leu359
  Accession # P35343
• Specificity: Stains mouse CXCR2-transfected cells but not CXCR1 transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR2/IL-8RB Antibody (242216) [Phycoerythrin]

• CD182 antigen
• CD182
• CDw128b
• chemokine (C-X-C motif) receptor 2
• chemokine (CXC) receptor 2
• CMKAR2
• C-X-C chemokine receptor type 2
• CXCR2 gene for IL8 receptor type B
• CXCR2
• CXC-R2
• CXCR-2
• GRO/MGSA receptor
• High affinity interleukin-8 receptor B
• IL-8 RB
• IL-8 receptor type 2
• IL-8R B
• IL8R2
• IL8RA
• IL8RB
• interleukin 8 receptor B
• interleukin 8 receptor type 2
• interleukin-8 receptor type B

Background

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha , GRO beta , GRO gamma , NAP-2, and ENA-78.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR2/IL-8RB Antibody (FAB2164P)(24)

Publications using FAB2164P

• FH Brennan, T Jogia, ER Gillespie, LV Blomster, XX Li, B Nowlan, GM Williams, E Jacobson, GW Osborne, FA Meunier, SM Taylor, KE Campbell, KP MacDonald, JP Levesque, TM Woodruff, MJ Ruitenberg Complement receptor C3aR1 controls neutrophil mobilization following spinal cord injury through physiological antagonism of CXCR2 JCI Insight, 2019-05-02;4(9):. 2019-05-02 [PMID: 31045582] (Flow Cytometry, Mouse)
• Vinit Kumar, Laxminarasimha Donthireddy, Douglas Marvel, Thomas Condamine, Fang Wang, Sergio Lavilla-Alonso et al. Cancer-Associated Fibroblasts Neutralize the Anti-tumor Effect of CSF1 Receptor Blockade by Inducing PMN-MDSC Infiltration of Tumors Cancer Cell 11/1/2017 [PMID: 29136508]
• J Xu, J Guardado, R Hoffman, H Xu, R Namas, Y Vodovotz, L Xu, M Ramadan, J Brown, HR Turnquist, TR Billiar IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model PLoS Med., 2017-07-25;14(7):e1002365. 2017-07-25 [PMID: 28742815] (Flow Cytometry, Mouse)
• X Wu, KK Dayanand, RP Thylur, CC Norbury, DC Gowda Small molecule-based inhibition of MEK1/2 proteins dampens inflammatory responses to malaria, reduces parasite load, and mitigates pathogenic outcomes J. Biol. Chem., 2017-07-05;0(0):. 2017-07-05 [PMID: 28679535] (Flow Cytometry, Mouse)
• D Wang, H Sun, J Wei, B Cen, RN DuBois CXCL1 is critical for pre-metastatic niche formation and metastasis in colorectal cancer Cancer Res., 2017-04-28;0(0):. 2017-04-28 [PMID: 28455419] (Flow Cytometry, Mouse)
• WB Lee, JJ Yan, JS Kang, Q Zhang, WY Choi, LK Kim, YJ Kim Mincle activation enhances neutrophil migration and resistance to polymicrobial septic peritonitis Sci Rep, 2017-01-23;7(0):41106. 2017-01-23 [PMID: 28112221] (Flow Cytometry, Mouse)
• A Broad-Spectrum Chemokine-Binding Protein of Bovine Papular Stomatitis Virus Inhibits Neutrophil and Monocyte Infiltration in Inflammatory and Wound Models of Mouse Skin PLoS ONE, 2016-12-09;11(12):e0168007. 2016-12-09 [PMID: 27936239] (Flow Cytometry, Mouse)
• Pedro Luiz Rosalen Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process Phytomedicine, 2016-09-28;23(13):1583-1590. 2016-09-28 [PMID: 27823622] (Flow Cytometry, Mouse)
• B7H3 ameliorates LPS-induced acute lung injury via attenuation of neutrophil migration and infiltration Sci Rep, 2016-08-12;6(0):31284. 2016-08-12 [PMID: 27515382] (Flow Cytometry, Mouse)
• Jia Li, Song Wu, Hongtu Tang, Wei Huang, Lushan Wang, Huanjiao Zhou et al. Long-Term Effects of Acupuncture Treatment on Airway Smooth Muscle in a Rat Model of Smoke-Induced Chronic Obstructive Pulmonary Disease Acupuncture in Medicine 4/1/2016 [PMID: 26345700]
• Citro A, Valle A, Cantarelli E, Mercalli A, Pellegrini S, Liberati D, Daffonchio L, Kastsiuchenka O, Ruffini P, Battaglia M, Allegretti M, Piemonti L CXCR1/2 inhibition blocks and reverses type 1 diabetes in mice. Diabetes, 2014-10-14;64(4):1329-40. 2014-10-14 [PMID: 25315007] (Flow Cytometry, Mouse)
• Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice. Mucosal Immunol, 2014-01-15;7(5):1079-93. 2014-01-15 [PMID: 24424522] (Flow Cytometry, Mouse)
• Wang , Penghua, Bai , Fengwei, Zenewicz , Lauren A, Dai , Jianfeng, Gate , David, Cheng , Gong, Yang , Long, Qian , Feng, Yuan , Xiaoling, Montgomery , Ruth R, Flavell , Richard, Town , Terrence, Fikrig , Erol IL-22 signaling contributes to West Nile encephalitis pathogenesis. PLoS ONE, 2012-08-28;7(8):e44153. 2012-08-28 [PMID: 22952908] (Flow Cytometry, Mouse)
• Ishii M, Asano K, Namkoong H, Tasaka S, Mizoguchi K, Asami T, Kamata H, Kimizuka Y, Fujiwara H, Funatsu Y, Kagawa S, Miyata J, Ishii K, Nakamura M, Hirai H, Nagata K, Kunkel SL, Hasegawa N, Betsuyaku T CRTH2 Is A Critical Regulator of Neutrophil Migration and Resistance to Polymicrobial Sepsis. J. Immunol., 2012-04-27;188(11):5655-64. 2012-04-27 [PMID: 22544936] (Flow Cytometry, Mouse)
• Spiller F, Carlos D, Souto FO, de Freitas A, Soares FS, Vieira SM, Paula FJ, Alves-Filho JC, Cunha FQ alpha-Acid glycoprotein decreases neutrophil migration and increases susceptibility to sepsis in diabetic mice. Diabetes, 2012-03-13;61(6):1584-91. 2012-03-13 [PMID: 22415874] (Flow Cytometry, Mouse)
• Salvucci O, Jiang K, Gasperini P MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1. Haematologica, 2012-01-22;97(6):818-26. 2012-01-22 [PMID: 22271895] (Flow Cytometry, Mouse)
• Chou RC, Kim ND, Sadik CD Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis. Immunity, 2010-08-27;33(2):266-78. 2010-08-27 [PMID: 20727790] (Flow Cytometry, Mouse)
• Zarbock A, Bishop J, Muller H, Schmolke M, Buschmann K, Van Aken H, Singbartl K Chemokine homeostasis vs. chemokine presentation during severe acute lung injury: the other side of the Duffy antigen receptor for chemokines. Am. J. Physiol. Lung Cell Mol. Physiol., 2010-01-08;298(3):L462-71. 2010-01-08 [PMID: 20061440] (Flow Cytometry, Mouse)
• Lee SC, Ju SA, Sung BH, Heo SK, Cho HR, Lee EA, Kim JD, Lee IH, Park SM, Nguyen QT, Suh JH, Kim BS Stimulation of the molecule 4-1BB enhances host defense against Listeria monocytogenes infection in mice by inducing rapid infiltration and activation of neutrophils and monocytes. Infect. Immun., 2009-02-23;77(5):2168-76. 2009-02-23 [PMID: 19237524] (Flow Cytometry, Mouse)
• Calderon B, Suri A, Miller MJ Dendritic cells in islets of Langerhans constitutively present beta cell-derived peptides bound to their class II MHC molecules. Proc. Natl. Acad. Sci. U.S.A., 2008-04-21;105(16):6121-6. 2008-04-21 [PMID: 18427107] (Flow Cytometry, Mouse)
• Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B Duffy antigen modifies the chemokine response in human endotoxemia. Crit. Care Med., 2008-01-01;36(1):159-65. 2008-01-01 [PMID: 18007272] (Flow Cytometry, Human)
• Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice. Eur. J. Immunol., 2007-10-01;37(10):2753-63. 2007-10-01 [PMID: 17823985] (Flow Cytometry, Mouse)
• Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling. Blood, 2007-04-01;109(7):2936-43. 2007-04-01 [PMID: 17132726] (Flow Cytometry, Human)
• Panopoulos AD, Zhang L, Snow JW, Jones DM, Smith AM, El Kasmi KC, Liu F, Goldsmith MA, Link DC, Murray PJ, Watowich SS STAT3 governs distinct pathways in emergency granulopoiesis and mature neutrophils. Blood, 2006-08-03;108(12):3682-90. 2006-08-03 [PMID: 16888100] (Flow Cytometry, Mouse)

Reviews for CXCR2/IL-8RB Antibody (FAB2164P) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 01/22/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 22952908
• Species: Mouse

reviewed by: Verified Customer Flow Mouse 01/22/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 22952908
• Species: Mouse

Bioinformatics

• Gene Symbol: CXCR2
• Uniprot:
  • P35343()