CXCR2/IL-8RB Antibody (242216) [Allophycocyanin]

Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F) and either (A) Rat Anti-Mouse CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB2164A) or (B) Rat IgG1Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB2164A). Quadrant markers were set based on control antibody staining (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 242216
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

CXCR2/IL-8RB Antibody (242216) [Allophycocyanin] Summary

• Immunogen: C6 rat glioma cell line transfected with mouse CXCR2
  Met1-Leu359
  Accession # P35343
• Specificity: Stains mouse CXCR2-transfected cells but not CXCR1 transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR2/IL-8RB Antibody (242216) [Allophycocyanin]

• CD182 antigen
• CD182
• CDw128b
• chemokine (C-X-C motif) receptor 2
• chemokine (CXC) receptor 2
• CMKAR2
• C-X-C chemokine receptor type 2
• CXCR2 gene for IL8 receptor type B
• CXCR2
• CXC-R2
• CXCR-2
• GRO/MGSA receptor
• High affinity interleukin-8 receptor B
• IL-8 RB
• IL-8 receptor type 2
• IL-8R B
• IL8R2
• IL8RA
• IL8RB
• interleukin 8 receptor B
• interleukin 8 receptor type 2
• interleukin-8 receptor type B

Background

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha , GRO beta , GRO gamma , NAP-2, and ENA-78.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR2/IL-8RB Antibody (FAB2164A)(5)

Publications using FAB2164A

• Jung, W;Yang, MJ;Kang, MS;Kim, JB;Yoon, KS;Yu, T;Yoon, C;Yang, HW;Choi, SJ;Park, EJ; Chronic lung tissue deposition of inhaled polyethylene microplastics may lead to fibrotic lesions Toxicology reports 2025-12-01 [PMID: 40860524] (Flow Cytometry, Mouse)
• Pruenster, M;Immler, R;Roth, J;Kuchler, T;Bromberger, T;Napoli, M;Nussbaumer, K;Rohwedder, I;Wackerbarth, LM;Piantoni, C;Hennis, K;Fink, D;Kallabis, S;Schroll, T;Masgrau-Alsina, S;Budke, A;Liu, W;Vestweber, D;Wahl-Schott, C;Roth, J;Meissner, F;Moser, M;Vogl, T;Hornung, V;Broz, P;Sperandio, M; E-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/S100A9 release from neutrophils via transient gasdermin D pore formation Nature immunology 2023-10-30 [PMID: 37903858] (Flow Cytometry, Mouse, Transgenic Mouse)
• Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice J Immunol, 2016-08-26;0(0):. 2016-08-26 [PMID: 27566826] (Flow Cytometry, Mouse)
• Citro A, Valle A, Cantarelli E, Mercalli A, Pellegrini S, Liberati D, Daffonchio L, Kastsiuchenka O, Ruffini P, Battaglia M, Allegretti M, Piemonti L CXCR1/2 inhibition blocks and reverses type 1 diabetes in mice. Diabetes, 2014-10-14;64(4):1329-40. 2014-10-14 [PMID: 25315007] (Flow Cytometry, Mouse)
• Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO J., 2012-07-31;31(17):3607-19. 2012-07-31 [PMID: 22850671] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CXCR2
• Uniprot:
  • P35343()