Human CXCL8/IL-8 ELISA Kit (Colorimetric)

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ELISA: Human CXCL8/IL-8 ELISA Kit (Colorimetric) [NBP1-83743] - Representative standard curve for human IL-8 ELISA. Human IL- 8 was diluted in serial 2-fold steps inSample Diluent. Do not use this standard curve to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, heparin)
Standard Curve Range
15.6 - 1,000 pg/ml
Sensitivity
2.0 pg/mL

Order Details

Human CXCL8/IL-8 ELISA Kit (Colorimetric) Summary

Specificity
The assay detects both natural and recombinant human IL-8/NAP-1. The interference of circulating factors of the immune system with high homology to IL-8/NAP-1 was evaluated by spiking these proteins at physiologically relevant concentrations (up to 1000 pg/ml) into a human IL-8/NAP-1 positive serum. There was no crossreactivity detected with connective tissue activating peptide 3, platelet factor 4 and neutrophil activating peptide 2 as well as IL-6, IL-2R, rhTNF beta and CD8.
Standard Curve Range
15.6 - 1,000 pg/ml
Sensitivity
2.0 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
8.7%
Intra-Assay
6.3%
Spike Recovery
The amount of endogenous human IL-8/NAP-1 in unspiked serum was subtracted from the spike values. The recovery ranged from 72% to 125% with an overall mean recovery of 88%.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL8

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Substrate Solution (tetramethyl-benzidine)
  2. Sample Diluent (Use when an external predilution of the samples is needed)
  3. Stop Solution (1M Phosphoric acid)
  4. Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
  5. Adhesive Films
  6. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-8/NAP-1, Biotin-Conjugate (anti-human IL-8/NAP-1 polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
  7. Aluminium pouch(es) with a human IL-8/NAP-1 Standard curve (coloured)

Alternate Names for Human CXCL8/IL-8 ELISA Kit (Colorimetric)

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAPGCP1
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

Interleukin-8 selectively stimulates the ability of neutrophils and T-lymphocytes to invade injured or inflamed tissue. IL-8 also known as CXCL8 that is purified from peripheral blood monocytes is a 10 kDa protein showing at least 4 variant forms (77, 72, 70, and 69 amino acids, respectively) which differ in the length of the N-terminal and readily form dimers in solution. Two cystin-bridges are essential for the biological activity of IL-8. Exogenous stimuli like LPS, but also IL-1, TNF-alpha and TNF-beta induce the secretion of IL-8 in a variety of different cell types including monocytes, endothelial and epithelial cells, peripheral blood mononuclear cells, dermal fibroblasts, keratinocytes, neutrophils, hepatocytes, synovial cells, and T-lymphocytes. The activities of IL-8 are not species-specific. Human IL-8 is also active in rodent and rabbit cells and differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. This chemokine activates multiple intracellular signaling pathways downstream of two cell-surface, G protein-coupled receptors (CXCR1 and CXCR2). The property of IL-8 to stimulate movement of neutrophils across endothelial monolayers in vitro supports the concept of a central role for this molecule in the accumulation of neutrophils at inflammatory lesions in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for CXCL8/IL-8 ELISA Kit (NBP1-83743). (Showing 1 - 1 of 1 FAQ).

  1. Can I purchase reagents of this kit separately?
    • Unfortunately, we can only offer the kit as sold. We are unable to offer any of the antibodies or reagents separately. Your best option (if you are looking to use the same capture antibody for your assay) may be to purchase all reagents separately and build your own kit. If you need assistance with putting this together, feel free to let us know.

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Bioinformatics

Gene Symbol IL8
Entrez