Human IL-8/CXCL8 Quantikine ELISA Kit


RT-qPCR for a set of cytokines (CSF2, IL6, IL8 and IL1B) and chemokines (CXCL1 and CXCL6) of total RNA samples derived from mono- and co-cultures in a transwell assay.The respective co- (+) or mono- (-) culture is more

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Human IL-8/CXCL8 Quantikine ELISA Kit Summary

The Quantikine Human IL-8 Immunoassay is a 3.5 hour solid phase ELISA designed to measure human IL-8 in cell culture supernates, serum, and plasma. It is based on antibodies raised against the 72 aa variant of human IL-8 derived from E. coli. It is calibrated with the same recombinant factor. This immunoassay accurately quantitates recombinant human IL-8. Measurement of natural huma...n IL-8 or the 77 aa variant of human IL-8 gave results parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.
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Natural and recombinant human IL-8
Assay Type
Solid Phase Sandwich ELISA
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Spike Recovery
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Sample Volume
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Application Notes
No significant interference observed with available related molecules.
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D8000C in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-8/CXCL8 Quantikine ELISA Kit

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • member 8
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1


Interleukin-8 (IL-8), also known as IL-8, GCP-1, and NAP-1, is a heparin-binding 8-9 kDa member of the alpha, or CXC family of chemokines. There are at least 15 human CXC family members that all adopt a three beta -sheet/one alpha -helix structure. Most CXC chemokines show an N-terminal Glu-Leu-Arg (ELR) tripeptide motif. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is considered the most bio-active, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). Mature human IL-8 shares 65-69% amino acid (aa) identity with canine, feline, and porcine IL-8. There is no IL-8 gene counterpart in rodent. 

Multiple isoforms of IL-8 are generated through both alternative splicing and differential proteolytic cleavage. In humans, alternative splicing generates an iso-form with an eleven aa substitution at the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8 and is likely a cell-specific event. For example, fibroblasts and endothelial cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes and lymphocytes generate the 6-77 form by cleaving following Leu25. These truncated forms generally show increased bioactivity, particularly through the CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor, a modification that increases its half-life and ability to induce leukocytosis. A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II great alveolar cells, and endothelial cells. 

IL-8 bioactivity is mediated through two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate into functional homodimers. They can also heterodimerize, but these complexes dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8 and is principally associated with chemotaxis and MMP-9 release. CXCR1, in contrast, responds to high concentrations of IL-8 and is associated with respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8 interaction with CXCR1. 

In addition to its pro-inflammatory effects, IL-8 is involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It induces VEGF expression in vascular endothelial cells and functions as an autocrine factor for EC growth and angiogenesis. It is upregulated in atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid following myocardial infarction. In cancer, IL-8 promotes epithelial-mesenchymal transition as well as tumor cell invasiveness and metastasis.
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Publications for CXCL8/IL-8 (D8000C)(434)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Bacteria - Streptococcus pyogenes, Bovine, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon), Primate - Papio hamadryas (Hamadyras Baboon), Rabbit,  Human.

We have publications tested in 1 application: ELISA Capture.

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ELISA Capture
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Bacteria - Streptococcus pyogenes
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca mulatta (Rhesus Macaque)
Primate - Papio cynocephalus (Yellow Baboon)
Primate - Papio hamadryas (Hamadyras Baboon)
All Species
Showing Publications 1 - 10 of 434. Show All 434 Publications.
Publications using D8000C Applications Species
Jin, Y;Zhang, D;Deng, K;Wu, P;Yang, D;Xie, Z;Qiu, W;Yu, G; Role of the cAMP-PKA-NF-?B pathway in Mucin1 over-expression in A549 cells during Respiratory syncytial virus infection BMC infectious diseases 2023-11-30 [PMID: 38036963] (Human) Human
Ur Rahim, J;Faheem, MM;Nawaz, S;Goswami, A;Rai, R; Synthesis and characterization of piperic acid conjugates with homochiral and heterochiral dipeptides containing phenylalanine and their application in skin cancer Peptides 2023-11-03 [PMID: 37923167] (Mouse) Mouse
Cho, YE;Kim, Y;Kim, SJ;Lee, H;Hwang, S; Overexpression of Interleukin-8 Promotes the Progression of Fatty Liver to Nonalcoholic Steatohepatitis in Mice International journal of molecular sciences 2023-10-23 [PMID: 37895168] (Human) Human
Kami?ska, J;Koper-Lenkiewicz, OM;Ponikwicka-Tyszko, D;Lebiedzi?ska, W;Palak, E;Sztachelska, M;Bernaczyk, P;Dorf, J;Guzi?ska-Ustymowicz, K;Zar?ba, K;Wo?czy?ski, S;Rahman, NA;Dymicka-Piekarska, V; New Insights on the Progesterone (P4) and PGRMC1/NENF Complex Interactions in Colorectal Cancer Progression Cancers 2023-10-20 [PMID: 37894441] (Human) Human
Planchais, C;Molinos-Albert, LM;Rosenbaum, P;Hieu, T;Kanyavuz, A;Clermont, D;Prazuck, T;Lefrou, L;Dimitrov, JD;Hüe, S;Hocqueloux, L;Mouquet, H; HIV-1 treatment timing shapes the human intestinal memory B-cell repertoire to commensal bacteria Nature communications 2023-10-10 [PMID: 37816704] (Human) Human
Wright, CW;Li, N;Shaffer, L;Hill, A;Boyer, N;Alves, SE;Venkataraman, S;Biswas, K;Lieberman, LA;Mohammadi, S; Establishment of a 96-well transwell system using primary human gut organoids to capture multiple quantitative pathway readouts Scientific reports 2023-09-29 [PMID: 37773535] (Human) Human
Kim, HJ;Chang, HK;Lee, YM;Heo, K; Catecholamines Promote Ovarian Cancer Progression through Secretion of CXC-Chemokines International journal of molecular sciences 2023-09-14 [PMID: 37762405] ( Human)  Human
Aboussekhra, A;Alraouji, NN;Al-Mohanna, FH;Al-Khalaf, H; Ionizing radiation normalizes the features of active breast cancer stromal fibroblasts and suppresses their paracrine pro-carcinogenic effects Translational oncology 2023-09-04 [PMID: 37672859] (Human) Human
Roach, DR;Noël, B;Chollet-Martin, S;de Jode, M;Granger, V;Debarbieux, L;de Chaisemartin, L; Human Neutrophil Response to Pseudomonas Bacteriophage PAK_P1, a Therapeutic Candidate Viruses 2023-08-12 [PMID: 37632068] (Human) Human
Murase, H;Matsuo, Y;Denda, Y;Nonoyama, K;Kato, T;Aoyama, Y;Hayashi, Y;Imafuji, H;Saito, K;Morimoto, M;Ogawa, R;Takahashi, H;Mitsui, A;Kimura, M;Takiguchi, S; Upregulation of integrin‑linked kinase enhances tumor progression in gemcitabine‑resistant pancreatic cancer Oncology reports 2023-09-01 [PMID: 37477162] (Human) Human
Show All 434 Publications.

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Gene Symbol CXCL8