Human IL-8/CXCL8 Quantikine ELISA Kit

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Applications ELISA

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Human IL-8/CXCL8 Quantikine ELISA Kit Summary

The Quantikine Human IL-8 Immunoassay is a 3.5 hour solid phase ELISA designed to measure human IL-8 in cell culture supernates, serum, and plasma. It is based on antibodies raised against the 72 aa variant of human IL-8 derived from E. coli. It is calibrated with the same recombinant factor. This immunoassay accurately quantitates recombinant human IL-8. Measurement of natural huma...n IL-8 or the 77 aa variant of human IL-8 gave results parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.
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Natural and recombinant human IL-8
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
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Sample Volume
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Application Notes
No significant interference observed with available related molecules.
Read Publications using
D8000C in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-8/CXCL8 Quantikine ELISA Kit

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • member 8
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1


Interleukin-8 (IL-8), also known as IL-8, GCP-1, and NAP-1, is a heparin-binding 8-9 kDa member of the alpha, or CXC family of chemokines. There are at least 15 human CXC family members that all adopt a three beta -sheet/one alpha -helix structure. Most CXC chemokines show an N-terminal Glu-Leu-Arg (ELR) tripeptide motif. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is considered the most bio-active, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). Mature human IL-8 shares 65-69% amino acid (aa) identity with canine, feline, and porcine IL-8. There is no IL-8 gene counterpart in rodent. 

Multiple isoforms of IL-8 are generated through both alternative splicing and differential proteolytic cleavage. In humans, alternative splicing generates an iso-form with an eleven aa substitution at the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8 and is likely a cell-specific event. For example, fibroblasts and endothelial cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes and lymphocytes generate the 6-77 form by cleaving following Leu25. These truncated forms generally show increased bioactivity, particularly through the CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor, a modification that increases its half-life and ability to induce leukocytosis. A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II great alveolar cells, and endothelial cells. 

IL-8 bioactivity is mediated through two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate into functional homodimers. They can also heterodimerize, but these complexes dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8 and is principally associated with chemotaxis and MMP-9 release. CXCR1, in contrast, responds to high concentrations of IL-8 and is associated with respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8 interaction with CXCR1. 

In addition to its pro-inflammatory effects, IL-8 is involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It induces VEGF expression in vascular endothelial cells and functions as an autocrine factor for EC growth and angiogenesis. It is upregulated in atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid following myocardial infarction. In cancer, IL-8 promotes epithelial-mesenchymal transition as well as tumor cell invasiveness and metastasis.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for CXCL8/IL-8 (D8000C)(387)

We have publications tested in 11 confirmed species: Human, Mouse, Rat, Bacteria - Streptococcus pyogenes, Bovine, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon), Primate - Papio hamadryas (Hamadyras Baboon), Rabbit.

We have publications tested in 1 application: ELISA Capture.

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ELISA Capture
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Bacteria - Streptococcus pyogenes
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca mulatta (Rhesus Macaque)
Primate - Papio cynocephalus (Yellow Baboon)
Primate - Papio hamadryas (Hamadyras Baboon)
All Species
Showing Publications 1 - 10 of 387. Show All 387 Publications.
Publications using D8000C Applications Species
DB Rosen, EA Murphy, RS Gejman, A Capili, RL Friedlande, S Rand, KA Cagino, SM Glynn, KC Matthews, JM Kubiak, J Yee, M Prabhu, LE Riley, YJ Yang Cytokine response over the course of COVID-19 infection in pregnant women Cytokine, 2022;154(0):155894. 2022 [PMID: 35490452] (Human) Human
YJ Park, D Acosta, R Vassell, J Tang, S Khurana, CD Weiss, H Golding, M Zaitseva D-dimer and CoV-2 spike-immune complexes contribute to the production of PGE2 and proinflammatory cytokines in monocytes PloS Pathogens, 2022;18(4):e1010468. 2022 [PMID: 35385545] (Human) Human
M Uchida, T Suga, M Terada, T Isaka A pilot study: the relationship between salivary MCP-1 and IgA, and exercise performance in long-distance runners and sprinters Bmc Research Notes, 2022;15(1):118. 2022 [PMID: 35346356] (Human) Human
YH Lee, SJ Lin Chitosan/PVA Hetero-Composite Hydrogel Containing Antimicrobials, Perfluorocarbon Nanoemulsions, and Growth Factor-Loaded Nanoparticles as a Multifunctional Dressing for Diabetic Wound Healing: Synthesis, Characterization, and In Vitro/In Vivo Evaluation Pharmaceutics, 2022;14(3):. 2022 [PMID: 35335913] (Human) Human
M Smeda, KM Galler, M Woelflick, A Rosendahl, C Moehle, B Lenhardt, W Buchalla, M Widbiller Molecular Biological Comparison of Dental Pulp- and Apical Papilla-Derived Stem Cells International Journal of Molecular Sciences, 2022;23(5):. 2022 [PMID: 35269758] (Human) Human
HS Kim, JW Lim, H Kim Korean Red Ginseng Extract Inhibits IL-8 Expression via Nrf2 Activation in Helicobacter pylori-Infected Gastric Epithelial Cells Nutrients, 2022;14(5):. 2022 [PMID: 35268019] (Human) Human
CL Pouille, S Ouaza, E Roels, J Behra, M Tourret, R Molinié, JX Fontaine, D Mathiron, D Gagneul, B Taminiau, G Daube, R Ravallec, C Rambaud, JL Hilbert, B Cudennec, A Lucau-Dani Chicory: Understanding the Effects and Effectors of This Functional Food Nutrients, 2022;14(5):. 2022 [PMID: 35267932] (Human) Human
ZQ Zhang, ZP Guo, XY Lv, P Sörös, XX Wang, L Wang, CH Liu Effect and neural mechanisms of the transcutaneous vagus nerve stimulation for relapse prevention in patients with remitted major depressive disorder: protocol for a longitudinal study BMJ Open, 2022;12(2):e050446. 2022 [PMID: 35193903] (Human) Human
YC Liu, HT Hsiao, JC Wang, TC Wen, SL Chen TGF-beta1 in plasma and cerebrospinal fluid can be used as a biological indicator of chronic pain in patients with osteoarthritis PLoS ONE, 2022;17(1):e0262074. 2022 [PMID: 35061744] (Human) Human
A Resano, S Bhattachar, M Barajas, KV Do, R Aguado-Jim, D Rodríguez, R Palacios, NG Bazán Elovanoids Counteract Inflammatory Signaling, Autophagy, Endoplasmic Reticulum Stress, and Senescence Gene Programming in Human Nasal Epithelial Cells Exposed to Allergens Pharmaceutics, 2022;14(1):. 2022 [PMID: 35057008] (Human) Human
Show All 387 Publications.

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Gene Symbol CXCL8