CXCL8/IL-8 Antibody [Unconjugated]

Images

 
IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
IL-8/CXCL8 was detected in immersion fixed paraffin-embedded sections of human tonsil (surgically removed due to severe EBV-induced mononucleosis) using HumanIL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody ...read more
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

CXCL8/IL-8 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Specificity
Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant canine
IL-8/CXCL8, recombinant feline IL-8/CXCL8 and recombinant porcine IL-8/CXCL8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL8
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.1-0.5 ug/mL
Reviewed Applications
Read 4 Reviews rated 4.5
using
AF-208-NA in the following applications:

Publications
Read Publications using
AF-208-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody [Unconjugated]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAP
  • MONAPGCP1
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL8/IL-8 Antibody (AF-208-NA)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 5 applications: ELISA Development, IHC, IHC-P, In Vivo, Neutralization.


Filter By Application
ELISA Development
(1)
IHC
(2)
IHC-P
(2)
In Vivo
(1)
Neutralization
(3)
All Applications
Filter By Species
Human
(7)
Mouse
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF-208-NA Applications Species
P Ortiz-Mont, A Londoño-Va, JP Vernot Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line Cell Commun. Signal, 2017;15(1):17. 2017 [PMID: 28472950] (Neutralization, Human) Neutralization Human
LQ Siew, SY Wu, S Ying, CJ Corrigan Cigarette smoking increases bronchial mucosal IL-17A expression in asthmatics, which acts in concert with environmental aeroallergens to engender neutrophilic inflammation Clin. Exp. Allergy, 2017;0(0):. 2017 [PMID: 28211191] (IHC, Human) IHC Human
SH Ahn, H Park, YH Ahn, S Kim, MS Cho, JL Kang, YH Choi Necrotic cells influence migration and invasion of glioblastoma via NF-?B/AP-1-mediated IL-8 regulation Sci Rep, 2016;6(0):24552. 2016 [PMID: 27076368] (IHC-P, Human) IHC-P Human
Hardison MT, Galin FS, Calderon CE, Djekic UV, Parker SB, Wille KM, Jackson PL, Oster RA, Young KR, Blalock JE, Gaggar A The presence of a matrix-derived neutrophil chemoattractant in bronchiolitis obliterans syndrome after lung transplantation. J. Immunol., 2009;182(7):4423-31. 2009 [PMID: 19299743] (Neutralization, Human) Neutralization Human
Bendrik C, Dabrosin C Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo. J. Immunol., 2009;182(1):371-8. 2009 [PMID: 19109168] (IHC-P, Human) IHC-P Human
Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, Ohba M, Sueki H, Kokubu F, Hizawa N, Adachi M, Huang SK, Iijima M Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis. J. Invest. Dermatol., 2009;129(3):650-6. 2009 [PMID: 18830271] (In Vivo, Mouse) In Vivo Mouse
Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo. Photochem. Photobiol., 2004;80(2):231-5. 2004 [PMID: 15362934] (IHC, Human) IHC Human
Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation. J. Virol., 2003;77(3):1727-37. 2003 [PMID: 12525606] (Neutralization, Primate - Macaca mulatta (Rhesus Macaque)) Neutralization Primate - Macaca mulatta (Rhesus Macaque)
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome. J. Immunol., 2002;169(11):6515-21. 2002 [PMID: 12444162] (ELISA Development, Human) ELISA Development Human

Reviews for CXCL8/IL-8 Antibody (AF-208-NA) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Other.

Reviews using AF-208-NA:
Filter by Applications
WB
(2)
IF
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(3)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23150618
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23150618

Details

Detection NotesSee PMID 23150618

Comments

CommentsPublished: See PMID 23150618
 
reviewed by:
Anonymous
WB Other 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 19552626
SpeciesOther

Blocking

Blocking DetailsSee PMID 19552626

Details

Detection NotesSee PMID 19552626

Comments

CommentsPublished: See PMID 19552626
 
reviewed by:
Anonymous
IF Human 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19258324
SpeciesHuman

Blocking

Blocking DetailsSee PMID 19258324

Details

Detection NotesSee PMID 19258324

Comments

CommentsPublished: See PMID 19258324Used at 1:100, worked very well.
 
reviewed by:
Anonymous
ELISA Human 01/06/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID 11744674
SpeciesHuman

Blocking

Blocking DetailsSee PMID 11744674

Details

Detection NotesSee PMID 11744674

Comments

CommentsPublished: See PMID 11744674

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FAQs for CXCL8/IL-8 Antibody (AF-208-NA) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF208

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

 
Anonymous
01/06/2015
Application: WB
Species: Human

 
Anonymous
01/06/2015
Application: WB
Species: Other

 
Anonymous
01/06/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol IL8
Uniprot