CXCL7/Thymus Chemokine-1 Antibody [Unconjugated]

CXCL7/Thymus Chemokine-1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CXCL7/Thymus Chemokine-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Recombinant Mouse CXCL7/Thymus Chemo­kine‑1 (Catalog # 1091-CK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL7/Thymus Chemo­kine‑1 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL7/Thymus Chemo­kine‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793). The ND50 is typically 0.8-4 µg/mL.

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL7/Thymus Chemokine-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CXCL7/Thymus Chemokine-1
  Lys40-Tyr113
  Accession # AAG36786
• Specificity: Detects CXCL7/Thymus Chemokine‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant rat CXCL7/Thymus Chemokine-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ppbp
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.8-4 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL7/Thymus Chemokine-1 Antibody [Unconjugated]

• Beta-TG;B-TG1;CTAP3;CTAPIII;CTAP-III;CXCL7;LA-PF4;LDGF;MDGF;NAP-2;PBP;Platelet basic protein;PPBP;SCYB7;TC1;TC2;TGB;TGB1;THBGB;THBGB1
• Thymus Chemokine1
• Thymus Chemokine-1

Background

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein. Signal P prediction program analysis indicates that this precursor protein contains an amino terminal 39 amino acid residue signal peptide and a 74 amino acid mature chemokine. Mouse CXCL7 shares 72% amino acid sequence identity with rat CXCL7. The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract mouse BaF3 cells transfected with human CXCR2, but not human CXCR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL7/Thymus Chemokine-1 Antibody (AF793)(3)

Publications using AF793

• Chen Hao Lo, Gemma Shay, Jeremy J. McGuire, Tao Li, Kenneth H. Shain, Jun Yong Choi et al. Host-Derived Matrix Metalloproteinase-13 Activity Promotes Multiple Myeloma–Induced Osteolysis and Reduces Overall Survival Cancer Research 2021-05-01 [PMID: 33526510]
• Yoh Goto CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow Stem Cells, 2016-07-08;0(0):. 2016-07-08 [PMID: 27339271] (Neutralization, Mouse)
• Wang H, Hong L, Huang J, Jiang Q, Tao R, Tan C, Lu N, Wang C, Ahmed M, Lu Y, Liu Z, Shi W, Lai E, Wilcox C, Han F P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy. Cell Res, 2015-05-22;25(6):674-90. 2015-05-22 [PMID: 25998681] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: Ppbp
• Uniprot:
  • AAG36786()