Recombinant Rat CXCL7/NAP-2 Protein

SDS-Page: Rat CXCL7/NAP-2 Protein [NBP2-35279]

• Reactivity: Rt
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Rat CXCL7/NAP-2 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 62 amino acids corresponding to CXCL7/NAP-2 Source: E. coli

  Amino Acid Sequence: IELRCRCTNT LSGIPLNSIS RVNVFRPGAH CDNVEVIATL KNGKEVCLDP TAPMIKKIVK KI

• Details of Functionality: NAP2 Protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.1-1.0 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PPBP
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CXCL7/NAP-2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 6.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rat CXCL7/NAP-2 Protein

• Beta-TG
• connective tissue-activating peptide III
• CTAP III
• CTAP3CTAP-III
• CTAPIII
• CXC chemokine ligand 7
• C-X-C motif chemokine 7
• CXCL7
• CXCL7b-TG1
• LA-PF4
• LDGFTC1
• Leukocyte-derived growth factor
• low-affinity platelet factor IV
• Macrophage-derived growth factor
• MDGFTC2
• NAP2
• NAP-2
• NAP-2/CXCL7
• NAP-2-L1
• neutrophil-activating peptide 2
• neutrophil-activating peptide-2
• PBPTHBGB
• platelet basic protein
• PPBP
• pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
• SCAR10
• SCYB7beta-thromboglobulin
• small inducible cytokine B7
• small inducible cytokine subfamily B, member 7
• Small-inducible cytokine B7
• TGB
• TGB1B-TG1
• THBGB1
• thrombocidin 1
• thrombocidin 2
• thromboglobulin, beta-1

Background

CXCL7 is a small cytokine which is also an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein belonging to the CXC chemokine family and it is released in large amounts from platelets following their activation. CXCL7 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. Recombinant Rat CXCL7 contains 62 amino acids which is a single non-glycosylated polypeptide chain. In addition, The rat CXCL7 shares 58 % and 77 % a.a. sequence identity with human and mouse CXCL7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CXCL7/NAP-2 Recombinant Protein (NBP2-35279)(1)

Publication using NBP2-35279

• Hwang Y, Park J, Cho D Activation of Adenosine Monophosphate-activated Protein Kinase by Telmisartan Decreases Basal and Platelet-derived Growth Factor-stimulated Vascular Smooth Muscle Cell Proliferation via Inhibiting the Mammalian Target of Rapamycin/p70 S6 Kinase Signaling J Korean Med Sci 2020-08-07 (Rat)

Bioinformatics

• Gene Symbol: PPBP