CXCL13/BLC/BCA-1 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC203102 and based on accession number NM_006419. The protein contains a C-terminal DDK tag.
Homo sapiens chemokine (C-X-C motif) ligand 13 (CXCL13), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for CXCL13/BLC/BCA-1
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for CXCL13/BLC/BCA-1 Overexpression Lysate (Native)
- B cell-attracting chemokine 1
- B lymphocyte chemoattractant
- BCA1B-cell chemoattractant
- BCA-1CXC chemokine BLC
- B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
- BLCSmall-inducible cytokine B13
- B-lymphocyte chemoattractant
- chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
- chemokine (C-X-C motif) ligand 13
- C-X-C motif chemokine 13
- SCYB13B-cell-attracting chemokine 1
- small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)
B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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Publications for CXCL13/BLC/BCA-1 Lysate (NBL1-09624) (0)
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