Human CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1875
    • Availability
      Product Discontinued

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Human CXCL12/SDF-1 alpha ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard Curve. SDF-1alpha (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SDF-1alpha.
Sensitivity
10 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CXCL12

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Useful for quantification.
Publications
Read Publications using KA1875.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): Diluent buffer with 0.09% sodium azide as preservative.
  2. Assay Diluent B (Item E): 5x concentrated buffer.
  3. Detection Antibody SDF-1 alpha (Item F): Biotinylated anti-human SDF-1 alpha. Eachvial is enough to assay half microplate.
  4. HRP-Streptavidin concentrates (Item G): 200x concentrated HRP-conjugatedStreptavidin.
  5. SDF-1 alpha Microplate (Item A): 96 wells coated with anti-human SDF-1 alpha.
  6. Standard Protein (Item C): Human SDF-1 alpha, 1 vial is enough to rum eachstandard in duplicate.
  7. Stop Solution (Item I): 0.2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) inbuffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)

  • CXCL12 chemokine (C-X-C motif) ligand 12
  • CXCL12/SDF-1 alpha
  • IRH
  • PBSF
  • SCYB12
  • SDF1 alpha
  • SDF1
  • SDF1a
  • SDF1B
  • TLSF
  • TPAR1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for CXCL12/SDF-1 alpha ELISA Kit (KA1875)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CXCL12/SDF-1 alpha ELISA Kit (KA1875). (Showing 1 - 1 of 1 FAQs).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol CXCL12