KLHL2 Antibody (OTI1G7)

Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] Analysis of extracts (35ug) from 9 different cell lines by using anti-KLHL2 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: KLHL2 Antibody (1G7) [NBP2-02939] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2.

Flow Cytometry: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KLHL2 antibody, and then analyzed by flow cytometry.

Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF
• Clone: OTI1G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.7 mg/ml

KLHL2 Antibody (OTI1G7) Summary

• Description: Novus Biologicals Mouse KLHL2 Antibody (OTI1G7) (NBP2-02939) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. Anti-KLHL2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 (NP_009177) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLHL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 65.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.7 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KLHL2 Antibody (OTI1G7)

• ABP-KELCH
• Actin-binding protein Mayven
• kelch (Drosophila)-like 2 (Mayven)
• kelch
• kelch-like 2, Mayven (Drosophila)
• kelch-like protein 2
• MAV
• MAYVEN

Background

KLHL2, also known as Kelch-like protein 2, has a 593 short amino acid that is 66 kDa and a long 597 amino acid that is 67 kDa, predominantly expressed in brain, and may participate in organizing the actin cytoskeleton of the brain cells. This protein is currently being studied for research on the following diseases and disorders: contagious pustular dermatitis, lumpy skin disease, monkeypox, smallpox, cowpox, vaccinia, skin disease, dermatitis, pharyngitis, breast cancer, and neuronitis. The protein has been shown to interact with KLHL12 in intracellular protein transport pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KLHL2 Antibody (NBP2-02939)(1)

Publication using NBP2-02939

• Zhang E, Dong X, Chen S et al. Ubiquitin ligase KLHL2 promotes the degradation and ubiquitination of ARHGEF7 protein to suppress renal cell carcinoma progression Am J Cancer Res 2020-10-01 [PMID: 33163274] (WB, Human)

Bioinformatics

• Gene Symbol: KLHL2
• Entrez:
  • 11275(Human)