KLHL2 Antibody (OTI1G7)

Images

 
Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] Analysis of extracts (35ug) from 9 different cell lines by using anti-KLHL2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: KLHL2 Antibody (1G7) [NBP2-02939] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2.
Flow Cytometry: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KLHL2 antibody, and then analyzed ...read more
Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI1G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.7 mg/ml

Order Details

KLHL2 Antibody (OTI1G7) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 (NP_009177) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
KLHL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
Theoretical MW
65.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-02939 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.7 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KLHL2 Antibody (OTI1G7)

  • ABP-KELCH
  • Actin-binding protein Mayven
  • kelch (Drosophila)-like 2 (Mayven)
  • kelch
  • kelch-like 2, Mayven (Drosophila)
  • kelch-like protein 2
  • MAV
  • MAYVEN

Background

KLHL2, also known as Kelch-like protein 2, has a 593 short amino acid that is 66 kDa and a long 597 amino acid that is 67 kDa, predominantly expressed in brain, and may participate in organizing the actin cytoskeleton of the brain cells. This protein is currently being studied for research on the following diseases and disorders: contagious pustular dermatitis, lumpy skin disease, monkeypox, smallpox, cowpox, vaccinia, skin disease, dermatitis, pharyngitis, breast cancer, and neuronitis. The protein has been shown to interact with KLHL12 in intracellular protein transport pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KLHL2 Antibody (NBP2-02939)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol KLHL2
Entrez