CTLA-4 Antibody (1003705) [Alexa Fluor® 350]

• Reactivity: Hu, Mu
• Applications: WB, Func
• Clone: 1003705
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

CTLA-4 Antibody (1003705) [Alexa Fluor® 350] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human CTLA-4
  Ala37-Phe162
  Accession # P16410
• Specificity: Detects human CTLA-4 in direct ELISAs. Detects human and moue CTLA-4 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Agonist Activity
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CTLA-4 Antibody (1003705) [Alexa Fluor® 350]

• CD
• CD152 antigen
• CD152
• CD152IDDM12
• CD28
• celiac disease 3
• CELIAC3
• CTLA4
• CTLA-4
• cytotoxic T-lymphocyte antigen 4
• cytotoxic T-lymphocyte protein 4
• Cytotoxic T-lymphocyte-associated antigen 4
• cytotoxic T-lymphocyte-associated protein 4
• cytotoxic T-lymphocyte-associated serine esterase-4
• GRD4
• GSE
• ICOS
• ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Background

CTLA-4 (Cytotoxic T-lymphocyte Associated protein‑4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily (1, 2). Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41-43 kDa (2) Within the ECD, human CTLA-4 shares 68%, 71% and 83‑86% aa sequence identity with mouse, rat and porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3-5). Isoforms of 56-79 aa that mainly contain parts of the cytoplasmic domain are reported. In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4 (6). CD28, which is structurally related to CTLA-4, is constitutively expressed on naïve T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) (1, 7, 8). In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1-2 days after T cell activation (2, 7, 8). It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation (9). Abatacept, a therapeutic human CTLA-4-Ig fusion protein (trade name Orencia), competes with CD28 for B7-1 and B7-2 binding and has been used to antagonize T cell activation in autoimmune conditions and to enhance transplant survival (10). Mice deleted for CTLA-4 show no abnormalities until after birth, but then develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells, which constitutively express CTLA-4 in wild-type mice and humans (11-13).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.